PAWP CRISPR/Cas9 KO Plasmid (h): sc-406196
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAWP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAWP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAWP CRISPR/Cas9 KO Plasmid (h) | sc-406196 | 20 µg | $397.00 |
Overview
WBP2NL encodes PAWP (postacrosomal WW domain-binding protein), a sperm-borne factor enriched in the postacrosomal sheath that contributes to oocyte activation at fertilization. PAWP contains PPXY motifs that engage WW domain–containing signaling adaptors, linking it to phosphorylation-dependent regulatory networks implicated in early embryonic calcium oscillations and cell-cycle resumption. Functional studies associate altered PAWP expression or activity with impaired fertilization, abnormal embryonic development, and certain forms of male-factor infertility. As a germ cell–specific regulator, WBP2NL provides a tractable model for dissecting sperm–egg signaling, gamete quality control, and reproductive pathway crosstalk.
PAWP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the WBP2NL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the WBP2NL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the WBP2NL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAWP protein expression.
This CRISPR knockout system enables efficient generation of WBP2NL-deficient cell models for investigation of PAWP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting WBP2NL exon(s) critical for PAWP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple WBP2NL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAWP CRISPR/Cas9 KO Plasmid (h) and PAWP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the WBP2NL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAWP HDR Plasmid (h) and PAWP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by WBP2NL homology arms to support homology-directed repair at defined WBP2NL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.