Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PATZ1 Double Nickase Plasmid (h): sc-404881-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PATZ1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PATZ1 Double Nickase Plasmid (h) and PATZ1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PATZ1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PATZ1 Antibody (H-2): sc-393223
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PATZ1 Double Nickase Plasmid (h) | sc-404881-NIC | 20 µg | $410.00 | |||
PATZ1 Double Nickase Plasmid (h2) | sc-404881-NIC-2 | 20 µg | $410.00 |
PATZ1 (POZ/BTB and AT-hook-containing zinc finger protein 1) is a nuclear transcription factor that binds AT-rich DNA through zinc finger and AT-hook motifs and regulates gene expression programs controlling proliferation, differentiation, and apoptosis. It functions within chromatin-associated regulatory networks by modulating transcriptional repression and activation, including crosstalk with p53-dependent responses and other developmental transcriptional circuits. Dysregulated PATZ1 expression or activity has been linked to altered cell fate decisions and genomic stability, with reported associations across multiple tumor contexts and neurodevelopmental phenotypes. These properties make PATZ1 a useful target for dissecting transcriptional control, lineage specification, and stress-response pathways in human cell models.
PATZ1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PATZ1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PATZ1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PATZ1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PATZ1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.