PARP-14 Antibody (C-1): sc-377150
- PARP-14 Antibody (C-1) is a mouse monoclonal IgG1 κ PARP-14 antibody, cited in 12 publications, provided at 200 µg/ml
- raised against amino acids 291-358 mapping within an internal region of PARP-14 of human origin
- PARP-14 Antibody (C-1) is recommended for detection of PARP-14 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PARP-14 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-377150 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PARP-14 (C-1): sc-377150.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PARP-14 Antibody (C-1). This work is in progress.
PARP-14 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 291-358 of human PARP-14, with reactivity in mouse, rat, and human samples, making anti-PARP-14 antibody (C-1) suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PARP-14, also called 1600029O10Rik, ARTD8, CoaSt6, and mKIAA1268, is a member of the poly(ADP-ribose) polymerase (PARP) family, which is involved in post-translational modifications essential for regulating various cellular processes, including DNA repair, gene expression, and cell survival. One key post-translational modification catalyzed by PARP-14 is poly(ADP-ribosyl)ation, where ADP-ribose units are transferred from NAD+ to target proteins. This modification can lead to the formation of poly(ADP-ribose) chains, which significantly alter the function of the modified proteins. For example, poly(ADP-ribosyl)ation of histones can loosen chromatin structure, facilitating access to DNA repair machinery. PARP-14 also influences cellular stress responses and survival, particularly in cells under genotoxic stress. The role of PARP-14 in modifying proteins through poly(ADP-ribosyl)ation is vital for maintaining cellular homeostasis and promoting cell survival, as this process can prevent cell death pathways from being activated in damaged cells. This post-translational modification is crucial for PARP-14′s ability to modulate cellular functions and maintain genomic integrity, highlighting PARP-14′s importance in various biological processes. PARP-14 monoclonal antibody (C-1) serves as a reliable and versatile tool, empowering researchers to elucidate the pivotal roles of PARP-14 in DNA repair, gene regulation, and cell survival. By facilitating detailed studies of cellular homeostasis and genomic integrity across multiple species and diverse experimental applications, anti-PARP-14 antibody (C-1) significantly advances our understanding of essential biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PARP-14 Antibody (C-1) References:
- Overexpression of dominant negative PARP interferes with tumor formation of HeLa cells in nude mice: evidence for increased tumor cell apoptosis in vivo. | Hans, MA., et al. 1999. Oncogene. 18: 7010-5. PMID: 10597301
- B-aggressive lymphoma family proteins have unique domains that modulate transcription and exhibit poly(ADP-ribose) polymerase activity. | Aguiar, RC., et al. 2005. J Biol Chem. 280: 33756-65. PMID: 16061477
- CDK-dependent activation of poly(ADP-ribose) polymerase member 10 (PARP10). | Chou, HY., et al. 2006. J Biol Chem. 281: 15201-7. PMID: 16455663
- Collaborator of Stat6 (CoaSt6)-associated poly(ADP-ribose) polymerase activity modulates Stat6-dependent gene transcription. | Goenka, S., et al. 2007. J Biol Chem. 282: 18732-9. PMID: 17478423
- The diverse biological roles of mammalian PARPS, a small but powerful family of poly-ADP-ribose polymerases. | Hassa, PO. and Hottiger, MO. 2008. Front Biosci. 13: 3046-82. PMID: 17981777
- Poly(ADP-ribose) polymerase 1 promotes tumor cell survival by coactivating hypoxia-inducible factor-1-dependent gene expression. | Elser, M., et al. 2008. Mol Cancer Res. 6: 282-90. PMID: 18314489
- Poly (ADP-ribose) polymerase activity regulates apoptosis in HeLa cells after alkylating DNA damage. | Liu, X., et al. 2008. Cancer Biol Ther. 7: 934-41. PMID: 18376143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP-14 Antibody (C-1) | sc-377150 | 200 µg/ml | $316.00 | |||
PARP-14 Antibody (C-1) X | sc-377150 X | 200 µg/0.1 ml | $316.00 | |||
PARP-14 Antibody (C-1) AC | sc-377150 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PARP-14 Antibody (C-1) HRP | sc-377150 HRP | 200 µg/ml | $316.00 | |||
PARP-14 Antibody (C-1) FITC | sc-377150 FITC | 200 µg/ml | $330.00 | |||
PARP-14 Antibody (C-1) PE | sc-377150 PE | 200 µg/ml | $343.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 488 | sc-377150 AF488 | 200 µg/ml | $357.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 546 | sc-377150 AF546 | 200 µg/ml | $357.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 594 | sc-377150 AF594 | 200 µg/ml | $357.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 647 | sc-377150 AF647 | 200 µg/ml | $357.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 680 | sc-377150 AF680 | 200 µg/ml | $357.00 | |||
PARP-14 Antibody (C-1) Alexa Fluor® 790 | sc-377150 AF790 | 200 µg/ml | $357.00 |