PARP1 Antibody (E-8): sc-74469
- PARP1 Antibody (E-8) is a mouse monoclonal IgG1 κ, cited in 24 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of PARP-1 of human origin
- recommended for detection of PARP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications (sc-74469 X, 200 µg/0.1 ml)
- See PARP1 (F-2): sc-8007 for PARP1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PARP1 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PARP1 Antibody (E-8) (see ordering information below).
PARP-1 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-300 at the N-terminus of human PARP-1 and is reactive with mouse, rat, and human samples, making anti-PARP-1 antibody (E-8) suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PARP-1, also called poly(ADP-ribose) polymerase-1, ARTD1, Adprt, and Parp1, is a nuclear DNA-binding protein that plays a critical role in DNA repair, chromatin remodeling, and apoptosis. Located predominantly in the nucleus, PARP-1 is essential for recognizing DNA damage, especially strand breaks, and signaling for repair by catalyzing the transfer of ADP-ribose units from NAD+ to target proteins, including histones and other chromatin-associated proteins. This nuclear localization positions PARP-1 at the heart of genomic maintenance, allowing rapid response to DNA damage and helping prevent mutations that could lead to chromosomal instability. In addition to repair functions, PARP-1 influences transcription and cell death pathways, as cleavage of PARP-1 during apoptosis generates an 89-kDa fragment that translocates to the cytoplasm, contributing to the apoptotic process. PARP-1′s ability to regulate both survival and death pathways underscores PARP-1′s importance in maintaining cellular homeostasis and genome integrity. In conclusion, PARP-1 monoclonal antibody (E-8) enables comprehensive investigation of PARP-1′s essential roles in DNA repair, chromatin remodeling, and apoptosis across multiple species and experimental platforms.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP1 Antibody (E-8) | sc-74469 | 200 µg/ml | $322.00 | |||
PARP1 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-539524 | 200 µg Ab; 10 µg BP | $361.00 | |||
PARP1 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-541544 | 200 µg Ab; 20 µg BP | $361.00 | |||
PARP1 Antibody (E-8) X | sc-74469 X | 200 µg/0.1 ml | $322.00 |