PARP1 Antibody (B-10): sc-74470

PARP-1 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-300 at the N-terminus of human PARP-1 and shows reactivity with mouse, rat, and human samples, making anti-PARP-1 antibody (B-10) suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PARP-1, also called poly(ADP-ribose) polymerase-1, ARTD1, Adprt, and Parp1, is a nuclear DNA-binding protein with a complex structure that includes a DNA-binding domain, an automodification domain, and a catalytic domain. The DNA-binding domain, characterized by zinc finger motifs, enables PARP-1 to detect DNA strand breaks, which is crucial for initiating DNA repair. The automodification domain plays a role in regulating protein-protein interactions, including PARP-1 self-regulation, while the catalytic domain transfers ADP-ribose units from NAD+ to target proteins, such as histones and other chromatin-associated proteins, to facilitate chromatin relaxation and access to the DNA repair machinery. This structured multi-domain organization is essential for PARP-1′s role in maintaining genomic stability, as PARP-1 ensures precise coordination of DNA damage detection, signaling, and repair. The integrity of this structure is critical, as disruptions in PARP-1 function can lead to chromosomal instability, higher rates of chromosome fusions, and aneuploidy, underscoring PARP-1′s importance in safeguarding genome integrity. PARP-1 monoclonal antibody (B-10) stands out as a robust and versatile tool, empowering researchers to explore the pivotal roles of PARP-1 in DNA repair, chromatin regulation, and genomic integrity across multiple species and experimental platforms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARP1 Antibody (B-10) has 73 citations in a variety of scientific publications.

Primary antibodies like Anti-PARP1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARP1 Antibody (B-10) Specifications

Alternate Product Name: PARP-1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: PARP-1

Epitope Species: human

Product Reactivity/Detection: PARP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 73 citations in various scientific publications

Available Conjugates/Forms for PARP1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred PARP1 antibody is PARP1 Antibody (F-2): sc-8007.