PARM-1 CRISPR/Cas9 KO Plasmid (h): sc-406775
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARM-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PARM-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARM-1 CRISPR/Cas9 KO Plasmid (h) | sc-406775 | 20 µg | $397.00 |
Overview
PARM1 encodes prostate androgen-regulated mucin-like protein 1 (PARM-1), an endoplasmic reticulum–associated, mucin-like protein implicated in the regulation of cellular stress adaptation and secretory pathway homeostasis. Reported activities link PARM-1 to unfolded protein response signaling and ER quality-control processes that influence cell survival, apoptosis sensitivity, and differentiation programs. PARM1 expression is responsive to hormonal and tissue-context cues and has been examined in epithelial biology and tumor-associated transcriptional profiles. These features make PARM1 a useful entry point for studying how ER-linked pathways shape proteostasis, growth control, and disease-relevant phenotypes in human model systems.
PARM-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PARM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PARM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PARM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PARM-1 protein expression.
This CRISPR knockout system enables efficient generation of PARM1-deficient cell models for investigation of PARM-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PARM1 exon(s) critical for PARM-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PARM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PARM-1 CRISPR/Cas9 KO Plasmid (h) and PARM-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PARM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PARM-1 HDR Plasmid (h) and PARM-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PARM1 homology arms to support homology-directed repair at defined PARM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.