PARG Double Nickase Plasmid (h): sc-404382-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PARG Double Nickase Plasmid (h) and PARG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PARG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARG Antibody (H-1): sc-398563
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARG Double Nickase Plasmid (h) | sc-404382-NIC | 20 µg | $410.00 |
Poly(ADP-ribose) glycohydrolase (PARG) is the principal enzyme that hydrolyzes poly(ADP-ribose) (PAR) chains, counterbalancing PARP-dependent PARylation during the DNA damage response. By controlling PAR turnover, PARG shapes chromatin dynamics, replication stress responses, and the resolution of PAR-dependent signaling assemblies that form at DNA lesions. PARG activity intersects with pathways governing single-strand break repair, replication fork stability, and genome integrity, and altered PAR metabolism has been linked to tumor-associated DNA repair phenotypes and neurodegeneration-relevant stress responses. As a central regulator of PARylation homeostasis, PARG is widely used as a node for dissecting PARP–PARG pathway wiring and PAR-driven protein recruitment mechanisms.
PARG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PARG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PARG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PARG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PARG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.