Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PAP-α CRISPR/Cas9 KO Plasmid (h): sc-403906
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAP-α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAP-α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAP-α CRISPR/Cas9 KO Plasmid (h) | sc-403906 | 20 µg | $397.00 |
Overview
PAPOLA encodes poly(A) polymerase alpha (PAP-α), a core catalytic component of the mRNA 3′-end processing machinery that adds poly(A) tails to pre-mRNAs following cleavage at polyadenylation sites. By controlling poly(A) tail length and coupling with cleavage and polyadenylation specificity factors, PAP-α influences mRNA stability, nuclear export, and translational efficiency, thereby shaping global gene expression programs. PAPOLA-dependent alternative polyadenylation contributes to transcript isoform diversity and can remodel post-transcriptional regulation during proliferation and differentiation. Dysregulated 3′-end processing and polyadenylation patterns are implicated in cancer biology and other disorders of gene-expression control, making PAP-α a useful node for mechanistic studies of RNA metabolism.
PAP-α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAPOLA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAPOLA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAPOLA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAP-α protein expression.
This CRISPR knockout system enables efficient generation of PAPOLA-deficient cell models for investigation of PAP-α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAPOLA exon(s) critical for PAP-α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAPOLA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAP-α CRISPR/Cas9 KO Plasmid (h) and PAP-α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAPOLA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAP-α HDR Plasmid (h) and PAP-α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAPOLA homology arms to support homology-directed repair at defined PAPOLA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.