Pannexin-1 Antibody (D-12): sc-515941
- Pannexin-1 Antibody (D-12) is a mouse monoclonal IgG1 κ Pannexin-1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 127-426 mapping at the C-terminus of Pannexin-1 of human origin
- Pannexin-1 Antibody (D-12) is recommended for detection of Pannexin-1 of human origin by WB, IP, IF and ELISA
- Anti-Pannexin-1 Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Pannexin-1 Antibody (D-12) for WB applications. This reagent is now offered in a bundle with Pannexin-1 Antibody (D-12) (see ordering information below).
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Pannexin-1 (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Pannexin-1 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pannexin-1 (D-12) antibody is available non-conjugated and in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Gap junctions are formed by hexameric protein groups called connexins for transporting low molecular weight proteins between cells. Connexins, present in all metazoan organisms, serve diverse functions from controlling cell growth and differentiation to conducting electricity in excitable tissues. Several mammalian cells with malignant phenotypes show decreased connexin expression and gap junction communication. The pannexin gene family encodes a second class of putative gap junction proteins. Pannexins are highly conserved in invertebrates and mammals, highlighting their importance in gap junctional coupling function. Mammalian Pannexin-1 and Pannexin-3 share close relations, while Pannexin-2 is more distantly related. Pannexin-1 is a transmembrane protein forming calcium-permeable gap junctions between adjacent cells and in the endoplasmic reticulum. In erythrocytes, Pannexin-1 forms a mechanosensitive ATP-permeable channel in the nonjunctional plasma membrane.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pannexin-1 Antibody (D-12) References:
- The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins. | Baranova, A., et al. 2004. Genomics. 83: 706-16. PMID: 15028292
- Pannexin membrane channels are mechanosensitive conduits for ATP. | Bao, L., et al. 2004. FEBS Lett. 572: 65-8. PMID: 15304325
- Expression and functions of neuronal gap junctions. | Söhl, G., et al. 2005. Nat Rev Neurosci. 6: 191-200. PMID: 15738956
- Evolution of gap junction proteins--the pannexin alternative. | Panchin, YV. 2005. J Exp Biol. 208: 1415-9. PMID: 15802665
- Site-specific and developmental expression of pannexin1 in the mouse nervous system. | Ray, A., et al. 2005. Eur J Neurosci. 21: 3277-90. PMID: 16026466
- Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium. | Locovei, S., et al. 2006. FEBS Lett. 580: 239-44. PMID: 16364313
- Cell-cell communication beyond connexins: the pannexin channels. | Barbe, MT., et al. 2006. Physiology (Bethesda). 21: 103-14. PMID: 16565476
- Pannexin 1 in erythrocytes: function without a gap. | Locovei, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 7655-9. PMID: 16682648
- Functional implications of calcium permeability of the channel formed by pannexin 1. | Vanden Abeele, F., et al. 2006. J Cell Biol. 174: 535-46. PMID: 16908669
- Pannexin-1 channels and their emerging functions in cardiovascular diseases. | Li, L., et al. 2015. Acta Biochim Biophys Sin (Shanghai). 47: 391-6. PMID: 25921414
- Pannexin 1 channels and ATP release in epilepsy: two sides of the same coin: The contribution of pannexin-1, connexins, and CALHM ATP-release channels to purinergic signaling. | Dossi, E. and Rouach, N. 2021. Purinergic Signal. 17: 533-548. PMID: 34495463
- Gasdermins and pannexin-1 mediate pathways of chemotherapy-induced cell lysis in hematopoietic malignancies. | Zhou, B., et al. 2022. Sci Signal. 15: eabl6781. PMID: 36538593
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pannexin-1 Antibody (D-12) | sc-515941 | 200 µg/ml | $322.00 | |||
Pannexin-1 Antibody (D-12): m-IgG1 BP-HRP Bundle | sc-531814 | 200 µg Ab; 20 µg BP | $361.00 | |||
Pannexin-1 Antibody (D-12) AC | sc-515941 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pannexin-1 Antibody (D-12) HRP | sc-515941 HRP | 200 µg/ml | $322.00 | |||
Pannexin-1 Antibody (D-12) FITC | sc-515941 FITC | 200 µg/ml | $336.00 | |||
Pannexin-1 Antibody (D-12) PE | sc-515941 PE | 200 µg/ml | $349.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 488 | sc-515941 AF488 | 200 µg/ml | $364.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 546 | sc-515941 AF546 | 200 µg/ml | $364.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 594 | sc-515941 AF594 | 200 µg/ml | $364.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 647 | sc-515941 AF647 | 200 µg/ml | $364.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 680 | sc-515941 AF680 | 200 µg/ml | $364.00 | |||
Pannexin-1 Antibody (D-12) Alexa Fluor® 790 | sc-515941 AF790 | 200 µg/ml | $364.00 |