Pancreatic Polypeptide CRISPR/Cas9 KO Plasmid (m): sc-422391
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pancreatic Polypeptide CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pancreatic Polypeptide genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pancreatic Polypeptide CRISPR/Cas9 KO Plasmid (m) | sc-422391 | 20 µg | $397.00 |
Overview
Mouse Ppy encodes pancreatic polypeptide, a peptide hormone produced by pancreatic PP (γ) cells that modulates gastrointestinal motility, gallbladder contraction, and exocrine pancreatic secretion while influencing appetite and energy homeostasis. Through signaling via neuropeptide Y receptor family members, pancreatic polypeptide integrates autonomic inputs with nutrient sensing to coordinate digestive and metabolic responses. Altered PP-cell function and pancreatic polypeptide dynamics have been associated with dysregulated glucose metabolism, obesity-related phenotypes, and pancreatic endocrine remodeling in models of metabolic stress. Ppy is therefore widely used as a marker of PP-cell identity and as a node for studying islet cell heterogeneity and gut–pancreas communication.
Pancreatic Polypeptide CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppy gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppy together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppy open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pancreatic Polypeptide protein expression.
This CRISPR knockout system enables efficient generation of Ppy-deficient cell models for investigation of Pancreatic Polypeptide signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppy exon(s) critical for Pancreatic Polypeptide function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppy genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pancreatic Polypeptide CRISPR/Cas9 KO Plasmid (m) and Pancreatic Polypeptide CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppy locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pancreatic Polypeptide HDR Plasmid (m) and Pancreatic Polypeptide HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppy homology arms to support homology-directed repair at defined Ppy target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.