Date published: 2025-9-20

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pan-Cytokeratin Antibody (HEP 111): sc-53401

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Datasheets
  • pan-Cytokeratin Antibody (HEP 111) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against HEP2 epithelial cell line of human origin
  • recommended for detection of broad range of Cytokeratin proteins of human origin by WB, IP and IF
  • See pan-Cytokeratin (C11): sc-8018 for pan-Cytokeratin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for pan-Cytokeratin Antibody (HEP 111). This work is in progress.

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    Pan-Cytokeratin Antibody (HEP 111) is a mouse monoclonal IgG1 antibody that detects pan-Cytokeratin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-pan-Cytokeratin antibody (HEP 111) targets a diverse group of intermediate filament proteins that are crucial for maintaining the structural integrity of epithelial cells. These proteins are essential for cellular differentiation and tissue specialization while serving as important biomarkers for various malignancies. For instance, the expression of specific cytokeratins can indicate the type of epithelial tissue involved in a tumor, with cytokeratins 10 and 13 being highly expressed in certain squamous cell carcinomas, while cytokeratin 18 is prevalent in many adenocarcinomas and basal cell carcinomas. Understanding the expression patterns of these cytokeratins is vital for accurate tumor characterization and can significantly influence treatment decisions, making pan-Cytokeratin (HEP 111) monoclonal antibody an invaluable tool in both research and clinical diagnostics.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    pan-Cytokeratin Antibody (HEP 111)

    sc-53401
    200 µg/ml
    $316.00

    How much sample do you recommend loading on the gel for Western blot detection using pan-Cytokeratin (HEP 111): sc-53401?

    Asked by: Germaine
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
    Date published: 2015-02-16
    Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength in Hep G2 and Caco-2 whole cell lysates. -SCBT QC
    Date published: 2013-04-17
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    pan-Cytokeratin Antibody (HEP 111) is rated 4.0 out of 5 by 2.
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