PALM2-AKAP2 CRISPR/Cas9 KO Plasmid (h): sc-407117

Overview

PALM2-AKAP2 is a human fusion gene that gives rise to a chimeric protein combining features of paralemmin-2 (PALM2), a membrane-associated phosphoprotein implicated in actin remodeling and neurite outgrowth, with A-kinase anchoring protein 2 (AKAP2), a scaffold that spatially organizes cAMP-dependent protein kinase A (PKA) signaling. By tethering kinase and phosphatase activities to defined subcellular microdomains, PALM2-AKAP2 is positioned to influence compartmentalized cAMP/PKA signaling, cytoskeletal dynamics, and membrane trafficking. Altered expression or rearrangement involving this locus has been explored in the context of dysregulated signaling and cellular architecture changes relevant to cancer biology and neurodevelopmental processes. As a scaffold-linked signaling node, it is useful for studying how localized kinase signaling integrates with adhesion, motility, and morphology programs.

PALM2-AKAP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PALM2-AKAP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PALM2-AKAP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PALM2-AKAP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PALM2-AKAP2 protein expression.

This CRISPR knockout system enables efficient generation of PALM2-AKAP2-deficient cell models for investigation of PALM2-AKAP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting PALM2-AKAP2 exon(s) critical for PALM2-AKAP2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple PALM2-AKAP2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by PALM2-AKAP2 CRISPR/Cas9 KO Plasmid (h) and PALM2-AKAP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PALM2-AKAP2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by PALM2-AKAP2 HDR Plasmid (h) and PALM2-AKAP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PALM2-AKAP2 homology arms to support homology-directed repair at defined PALM2-AKAP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.