PAK5 CRISPR/Cas9 KO Plasmid (h): sc-404073
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAK5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAK5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAK5 CRISPR/Cas9 KO Plasmid (h) | sc-404073 | 20 µg | $397.00 |
Overview
PAK5 (p21-activated kinase 5) is a serine/threonine kinase activated downstream of Rho-family GTPases such as Cdc42 and Rac, integrating signals that regulate cytoskeletal remodeling, neurite outgrowth, and cell survival. It localizes to mitochondria and other subcellular compartments where it can influence apoptosis-related signaling and stress responses. PAK5 participates in pathways governing cell motility and polarity through phosphorylation of cytoskeletal and signaling intermediates, linking extracellular cues to changes in adhesion and migration. Dysregulated PAK5 activity or expression has been associated with aberrant proliferation, invasion, and therapy resistance phenotypes in multiple cancer contexts and is also relevant to neurobiology due to its roles in neuronal development and synaptic function.
PAK5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAK5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAK5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAK5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAK5 protein expression.
This CRISPR knockout system enables efficient generation of PAK5-deficient cell models for investigation of PAK5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAK5 exon(s) critical for PAK5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAK5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAK5 CRISPR/Cas9 KO Plasmid (h) and PAK5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAK5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAK5 HDR Plasmid (h) and PAK5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAK5 homology arms to support homology-directed repair at defined PAK5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.