Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PAK4 Double Nickase Plasmid (h): sc-401895-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAK4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PAK4 Double Nickase Plasmid (h) and PAK4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PAK4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PAK4 Antibody (B-3): sc-390507
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAK4 Double Nickase Plasmid (h) | sc-401895-NIC | 20 µg | $410.00 | |||
PAK4 Double Nickase Plasmid (h2) | sc-401895-NIC-2 | 20 µg | $410.00 |
PAK4 encodes p21-activated kinase 4, a serine/threonine kinase that functions downstream of Rho-family GTPases such as Cdc42 to coordinate cytoskeletal remodeling, cell polarity, and directional migration. PAK4 signaling interfaces with pathways controlling focal adhesion turnover, actin dynamics, and pro-survival cues, influencing processes including adhesion-dependent growth and invasive behavior. Through phosphorylation of cytoskeletal and regulatory substrates, PAK4 contributes to regulation of apoptosis resistance and cell-cycle progression. Dysregulated PAK4 expression or activity has been associated with oncogenic signaling contexts and metastatic phenotypes, making it a frequent target in studies of tumor cell motility and survival mechanisms.
PAK4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PAK4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PAK4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PAK4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PAK4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.