PAK4 Antibody (B-3): sc-390507

PAK4 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAK4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PAK4 Antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAK4, a member of the serine/threonine kinase family, plays a crucial role in cellular signaling pathways, particularly in the regulation of cytoskeletal dynamics and cell motility. PAK4 is primarily located in the cytoplasm and interacts with GTP-bound forms of the small GTPases Rac1 and Cdc42, which are essential for actin filament organization. PAK4 interaction with these GTPases is vital for the formation of actin-enriched filopodia, structures that facilitate cell movement and communication. PAK4 is implicated in the activation of the c-Jun N-terminal kinase (JNK) pathway, which is involved in various cellular processes, including stress responses and apoptosis. PAK4 gene is located on human chromosome 19, with notably high expression in tissues such as the prostate, testis, and colon, highlighting PAK4′s potential significance in both normal physiology and disease states.

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PAK4 Antibody (B-3) References:

1. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
2. Regulation of KRAS-PAK4 axis by microRNAs in cancer.  |  Choudhry, ZS., et al. 2014. Curr Pharm Des. 20: 5275-8. PMID: 24479809
3. rac, a novel ras-related family of proteins that are botulinum toxin substrates.  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
4. The role of PAK4 in the immune system and its potential implication in cancer immunotherapy.  |  Naїja, A., et al. 2021. Cell Immunol. 367: 104408. PMID: 34246086
5. Targeting PAK4 reverses cisplatin resistance in NSCLC by modulating ER stress.  |  Liu, S., et al. 2024. Cell Death Discov. 10: 36. PMID: 38238316
6. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway.  |  Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
7. A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20.  |  Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
8. Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1.  |  Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
9. A brain serine/threonine protein kinase activated by Cdc42 and Rac1.  |  Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
10. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
11. A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.  |  Lange-Carter, CA., et al. 1993. Science. 260: 315-9. PMID: 8385802
12. PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia.  |  Abo, A., et al. 1998. EMBO J. 17: 6527-40. PMID: 9822598

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAK4 Antibody (B-3) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-PAK4 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAK4 Antibody (B-3) Specifications

Alternate Product Name: Serine/Threonine Kinase PAK-4

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 167-209

Epitope Target: PAK4

Epitope Species: human

Product Reactivity/Detection: PAK4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for PAK4 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin