PAI-RBP1 Antibody (F-8): sc-376832

PAI-RBP1 Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects PAI-RBP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PAI-RBP1 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAI-RBP1, also known as plasminogen activator inhibitor 1 RNA-binding protein, SERBP1, CGI-55, and CHD3IP, is a membrane-associated protein primarily localized in the nucleus, perinuclear region of the cytoplasm, and plasma membrane. PAI-RBP1 plays a crucial role in mRNA stability regulation, specifically binding to the cyclic nucleotide-responsive sequence motif of PAI-1 mRNA, which stabilizes mRNA and modulates expression. PAI-RBP1 interacts with Mi2-α, suggesting involvement in chromatin remodeling processes, and associates with PGRMC1, contributing to progesterone′s antiapoptotic effects in ovarian cells. PAI-RBP1 gene overexpression in ovarian cancer indicates potential roles in tumorigenesis and metastasis, making PAI-RBP1 a significant target for cancer biology research and therapeutic development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PAI-RBP1 Antibody (F-8) References:

1. Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the Type-1 plasminogen activator inhibitor mRNA.  |  Heaton, JH., et al. 2001. J Biol Chem. 276: 3341-7. PMID: 11001948
2. Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3.  |  Lemos, TA., et al. 2003. FEBS Lett. 533: 14-20. PMID: 12505151
3. Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries: PAIRBP1 regulation of granulosa and luteal cell viability.  |  Peluso, JJ., et al. 2005. Biol Reprod. 73: 261-70. PMID: 15814896
4. Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic action.  |  Peluso, JJ., et al. 2006. Endocrinology. 147: 3133-40. PMID: 16513825
5. CGI-55 interacts with nuclear proteins and co-localizes to p80-coilin positive-coiled bodies in the nucleus.  |  Lemos, TA. and Kobarg, J. 2006. Cell Biochem Biophys. 44: 463-74. PMID: 16679534
6. Ki-1/57 interacts with PRMT1 and is a substrate for arginine methylation.  |  Passos, DO., et al. 2006. FEBS J. 273: 3946-61. PMID: 16879614
7. LINE-1 ORF1 protein localizes in stress granules with other RNA-binding proteins, including components of RNA interference RNA-induced silencing complex.  |  Goodier, JL., et al. 2007. Mol Cell Biol. 27: 6469-83. PMID: 17562864
8. Expression analysis and RNA localization of PAI-RBP1 (SERBP1) in epithelial ovarian cancer: association with tumor progression.  |  Koensgen, D., et al. 2007. Gynecol Oncol. 107: 266-73. PMID: 17698176
9. Progesterone receptor membrane component-1 (PGRMC1) is the mediator of progesterone's antiapoptotic action in spontaneously immortalized granulosa cells as revealed by PGRMC1 small interfering ribonucleic acid treatment and functional analysis of PGRMC1 mutations.  |  Peluso, JJ., et al. 2008. Endocrinology. 149: 534-43. PMID: 17991724
10. The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation.  |  Kosti, A., et al. 2020. Genome Biol. 21: 195. PMID: 32762776
11. A genetically-encoded crosslinker screen identifies SERBP1 as a PKCε substrate influencing translation and cell division.  |  Martini, S., et al. 2021. Nat Commun. 12: 6934. PMID: 34836941
12. SERBP1 affects the apoptotic level by regulating the expression and alternative splicing of cellular and metabolic process genes in HeLa cells.  |  Zhou, J., et al. 2022. PeerJ. 10: e14084. PMID: 36213507

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAI-RBP1 Antibody (F-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PAI-RBP1 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAI-RBP1 Antibody (F-8) Specifications

Alternate Product Name: PAI-RBP1

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 139-173

Epitope Target: PAI-RBP1

Epitope Species: human

Product Reactivity/Detection: PAI-RBP1 isoforms 1-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PAI-RBP1 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin