PAC-1 Antibody (4O21) recommended for detection of PAC-1 of human origin by WB, IP, IF and IHC(P)

PAC-1 Antibody (4O21): sc-32776

PAC-1 Antibody (4O21) is rated 4.8 out of 5 by 8.
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Datasheets

    • Anti-PAC-1 Antibody (4O21) is a mouse monoclonal IgG2a (kappa light chain) PAC-1 antibody provided at 200 µg/ml
    • raised against recombinant PAC-1 of human origin
    • PAC-1 Antibody (4O21) is recommended for detection of PAC-1 of human origin by WB, IP, IF and IHC(P)
    • Anti-PAC-1 Antibody (4O21) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PAC-1 (4O21): sc-32776.

Can PAC-1 (4O21): sc-32776 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PAC-1 (4O21): sc-32776 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-04-04
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Rated 3 out of 5 by from No visible band in human airway smooth muscle No band in western from 15ug total protein using human airway smooth muscle cell lysates (1:200).
Date published: 2017-07-31
Rated 5 out of 5 by from used for WB p-akt1 antibody for WB use, I suggest diluted this antibody at 1:500.
Date published: 2017-02-24
Rated 5 out of 5 by from Excellent Antibody The PAC-1 Antibody (4O21): sc-32776 works very well for multiple applications including WB, IP, IF and IHC(P). This product has also been cited several times for further verification.
Date published: 2017-01-20
Rated 5 out of 5 by from Strong Strong, clean band in western blot analysis of PAC-1 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2015-07-06
Rated 5 out of 5 by from Used for western blotting with human ectopic Used for western blotting with human ectopic stromal cells. -SCBT Publication Review
Date published: 2015-06-22
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lateral ventricle tissue. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Positive experience Positive experience
Date published: 2015-04-09
Rated 5 out of 5 by from Published western blot data using human MB435 Published western blot data using human MB435 cell lysates. -SCBT Publication Review
Date published: 2015-02-06
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