PABPC2 CRISPR/Cas9 KO Plasmid (m): sc-422101
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PABPC2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PABPC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PABPC2 CRISPR/Cas9 KO Plasmid (m) | sc-422101 | 20 µg | $397.00 |
Overview
Pabpc2 encodes poly(A)-binding protein cytoplasmic 2 (PABPC2), an RNA-binding factor that associates with poly(A) tails to coordinate mRNA stability, translational efficiency, and turnover through interactions with translation initiation and mRNA decay machinery. As part of post-transcriptional gene regulation, PABPC2 contributes to dynamic control of gene expression programs during cellular stress responses, differentiation, and developmental processes in mouse. Altered poly(A) tail regulation and cytoplasmic mRNP remodeling are broadly linked to dysregulated proteostasis and aberrant RNA metabolism, which are common features in models of neurodegeneration, cancer biology, and immune signaling. Pabpc2 therefore serves as a useful node for interrogating pathways that couple mRNA fate decisions to cellular phenotype.
PABPC2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pabpc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pabpc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pabpc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PABPC2 protein expression.
This CRISPR knockout system enables efficient generation of Pabpc2-deficient cell models for investigation of PABPC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pabpc2 exon(s) critical for PABPC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pabpc2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PABPC2 CRISPR/Cas9 KO Plasmid (m) and PABPC2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pabpc2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PABPC2 HDR Plasmid (m) and PABPC2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pabpc2 homology arms to support homology-directed repair at defined Pabpc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.