PA28β Antibody (G-10): sc-390563
- PA28β Antibody (G-10) is a mouse monoclonal IgG1 λ PA28β antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 176-239 mapping at the C-terminus of PA28β of human origin
- PA28 beta Antibody (G-10) is recommended for detection of PA28β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
- Anti-PA28 beta Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for PA28β Antibody (G-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with PA28β Antibody (G-10) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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PA28β Antibody (G-10) is a mouse monoclonal IgG1 antibody that detects PA28 beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PA28β (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PA28 beta protein plays a crucial role as an interferon-γ (IFN-γ) inducible proteasome activator, which is essential for the effective presentation of certain major histocompatibility class I antigens. PA28 beta protein is located in the cytoplasm, where PA28 beta associates with the 20S proteasome to enhance proteolytic activity, thereby facilitating the degradation of ubiquitinated proteins and the generation of peptide fragments for MHC class I presentation. The PA28 complex, composed of PA28α and PA28β subunits, forms a hexameric ring structure that binds to both ends of the proteasome, significantly influencing proteasome function in antigen processing. Notably, PA28β expression is constitutive in antigen-presenting cells, and PA28β downregulation can lead to abnormal proteasome activation, which has been implicated in the development of intimal hyperplasia in various animal models. PA28β protein is also known by other names, including PSME2 and REG-β, highlighting PA28β importance in immune responses and potential implications in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PA28β Antibody (G-10) References:
- A second gene encoding the mouse proteasome activator PA28beta subunit is part of a LINE1 element and is driven by a LINE1 promoter. | Zaiss, DM. and Kloetzel, PM. 1999. J Mol Biol. 287: 829-35. PMID: 10222192
- Interferon gamma regulates accumulation of the proteasome activator PA28 and immunoproteasomes at nuclear PML bodies. | Fabunmi, RP., et al. 2001. J Cell Sci. 114: 29-36. PMID: 11112687
- Properties of the beta subunit of the proteasome activator PA28 (11S REG). | Wilk, S., et al. 2000. Arch Biochem Biophys. 384: 174-80. PMID: 11147828
- Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta. | Murata, S., et al. 2001. EMBO J. 20: 5898-907. PMID: 11689430
- Relationship of the 20S proteasome and the proteasome activator PA28 to atherosclerosis and intimal hyperplasia in the human vascular system. | Faries, PL., et al. 2001. Ann Vasc Surg. 15: 628-33. PMID: 11769143
- Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes. | Kohda, K., et al. 1998. J Immunol. 160: 4923-35. PMID: 9590240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PA28β Antibody (G-10) | sc-390563 | 200 µg/ml | $322.00 | |||
PA28β Antibody (G-10): m-IgGλ BP-HRP Bundle | sc-523584 | 200 µg Ab, 40 µg BP | $361.00 | |||
PA28β Antibody (G-10) AC | sc-390563 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PA28β Antibody (G-10) HRP | sc-390563 HRP | 200 µg/ml | $322.00 | |||
PA28β Antibody (G-10) FITC | sc-390563 FITC | 200 µg/ml | $336.00 | |||
PA28β Antibody (G-10) PE | sc-390563 PE | 200 µg/ml | $349.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 488 | sc-390563 AF488 | 200 µg/ml | $364.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 546 | sc-390563 AF546 | 200 µg/ml | $364.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 594 | sc-390563 AF594 | 200 µg/ml | $364.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 647 | sc-390563 AF647 | 200 µg/ml | $364.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 680 | sc-390563 AF680 | 200 µg/ml | $364.00 | |||
PA28β Antibody (G-10) Alexa Fluor® 790 | sc-390563 AF790 | 200 µg/ml | $364.00 |