Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p73 Double Nickase Plasmid (h): sc-416822-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p73 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p73 Double Nickase Plasmid (h) and p73 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TP73. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p73 Antibody (E-4): sc-17823
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p73 Double Nickase Plasmid (h) | sc-416822-NIC | 20 µg | $410.00 | |||
p73 Double Nickase Plasmid (h2) | sc-416822-NIC-2 | 20 µg | $410.00 |
TP73 encodes p73, a p53 family transcription factor that regulates cell-cycle control, apoptosis, and differentiation programs in response to cellular stress and developmental cues. Through isoform-specific activities, p73 influences DNA damage signaling, mitochondrial apoptosis, and transcriptional networks governing epithelial integrity and neural development. p73 function intersects with pathways such as ATM/ATR-mediated responses and p53 family cross-regulation, shaping outcomes including senescence and survival. Dysregulated TP73 expression or isoform balance has been associated with oncogenic processes, neurodevelopmental phenotypes, and altered stress adaptation, making it a useful node for mechanistic studies of transcriptional control.
p73 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TP73 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TP73. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TP73 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TP73-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.