Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p68 RNA Helicase CRISPR/Cas9 KO Plasmid (h): sc-402401
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p68 RNA Helicase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the p68 RNA Helicase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p68 RNA Helicase Antibody (D-7): sc-365164
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p68 RNA Helicase CRISPR/Cas9 KO Plasmid (h) | sc-402401 | 20 µg | $397.00 |
Overview
DDX5 encodes p68 RNA helicase, a DEAD-box ATP-dependent RNA helicase that remodels RNA and ribonucleoprotein complexes to control pre-mRNA splicing, ribosome biogenesis, and microRNA processing. p68 also acts as a transcriptional co-regulator by interfacing with signaling-responsive transcription factors, linking RNA metabolism to gene-expression programs governing cell-cycle progression and stress responses. Through these activities, DDX5 contributes to maintenance of genome integrity and coordination of DNA damage responses with RNA processing. Dysregulated DDX5 expression or function has been associated with oncogenic transcriptional networks and altered RNA splicing landscapes in multiple tumor contexts, making it a useful node for mechanistic studies of RNA-driven disease phenotypes.
p68 RNA Helicase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDX5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish p68 RNA Helicase protein expression.
This CRISPR knockout system enables efficient generation of DDX5-deficient cell models for investigation of p68 RNA Helicase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX5 exon(s) critical for p68 RNA Helicase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by p68 RNA Helicase CRISPR/Cas9 KO Plasmid (h) and p68 RNA Helicase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by p68 RNA Helicase HDR Plasmid (h) and p68 RNA Helicase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX5 homology arms to support homology-directed repair at defined DDX5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.