p68 RNA Helicase Antibody (A-5): sc-166167
- p68 RNA Helicase Antibody (A-5) is a mouse monoclonal IgG2a κ, cited in 15 publications, provided at 200 µg/ml
- raised against amino acids 471-614 mapping at the C-terminus of p68 RNA Helicase of human origin
- recommended for detection of p68 RNA Helicase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and bovine
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for p68 RNA Helicase Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p68 RNA Helicase Antibody (A-5) (see ordering information below).
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p68 RNA Helicase Antibody (A-5) is a mouse monoclonal IgG2a antibody that detects p68 RNA helicase (DDX5) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p68 RNA helicase (DDX5) is a crucial nuclear protein known for RNA-dependent ATPase activity, which is essential for various cellular processes, including RNA metabolism and gene expression regulation. p68 RNA helicase (DDX5) plays a significant role in organ differentiation during development, highlighting involvement in embryogenesis. p68 RNA helicase (DDX5) is expressed during early neural development and in various mesodermal tissues across different chordate embryos, indicating a vital role in developmental biology. Expression levels of p68 RNA helicase (DDX5) increase in serum-induced quiescent cell lines, suggesting potential involvement in cellular responses to growth signals. p68 RNA helicase (DDX5) may act as a coactivator for estrogen receptor alpha and associates with transcriptional coactivators such as CBP and p300, underscoring importance in transcriptional regulation. Under normal conditions, p68 RNA helicase (DDX5) localizes to the nucleus, and during late telophase, colocalizes with fibrillarin to form nascent nucleoli, indicating a role in ribosome biogenesis. p68 RNA helicase (DDX5) may function as a heterodimer with p72 RNA helicase, further expanding functional repertoire in cellular processes.
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p68 RNA Helicase Antibody (A-5) References:
- p68 RNA helicase is an essential human splicing factor that acts at the U1 snRNA-5' splice site duplex. | Liu, ZR. 2002. Mol Cell Biol. 22: 5443-50. PMID: 12101238
- Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300. | Rossow, KL. and Janknecht, R. 2003. Oncogene. 22: 151-6. PMID: 12527917
- Phosphorylation of p68 RNA helicase regulates RNA binding by the C-terminal domain of the protein. | Yang, L., et al. 2004. Biochem Biophys Res Commun. 314: 622-30. PMID: 14733953
- Bacterially expressed recombinant p68 RNA helicase is phosphorylated on serine, threonine, and tyrosine residues. | Yang, L. and Liu, ZR. 2004. Protein Expr Purif. 35: 327-33. PMID: 15135410
- ATPase/helicase activities of p68 RNA helicase are required for pre-mRNA splicing but not for assembly of the spliceosome. | Lin, C., et al. 2005. Mol Cell Biol. 25: 7484-93. PMID: 16107697
- P68 RNA helicase as a molecular target for cancer therapy. | Dai, TY., et al. 2014. J Exp Clin Cancer Res. 33: 64. PMID: 25150365
- Downregulation of p68 RNA Helicase (DDX5) Activates a Survival Pathway Involving mTOR and MDM2 Signals. | Kokolo, M. and Bach-Elias, M. 2017. Folia Biol (Praha). 63: 52-59. PMID: 28557706
- P68 RNA helicase promotes invasion of glioma cells through negatively regulating DUSP5. | Wang, R., et al. 2019. Cancer Sci. 110: 107-117. PMID: 30387548
- P68 RNA Helicase facilitates Breast Cancer progression by promoting Proliferation and Migration via PDGFR-β/AR axis. | Panchbhai, N., et al. 2021. J Cancer. 12: 6543-6552. PMID: 34659545
- P68 RNA Helicase (DDX5) Required for the Formation of Various Specific and Mature miRNA Active RISC Complexes. | Kokolo, M. and Bach-Elias, M. 2022. Microrna. 11: 36-44. PMID: 35184719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p68 RNA Helicase Antibody (A-5) | sc-166167 | 200 µg/ml | $316.00 | |||
p68 RNA Helicase Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-540034 | 200 µg Ab; 10 µg BP | $354.00 | |||
p68 RNA Helicase Antibody (A-5): m-IgG2a BP-HRP Bundle | sc-546840 | 200 µg Ab; 10 µg BP | $354.00 |