P5CR2 CRISPR/Cas9 KO Plasmid (h): sc-406048
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P5CR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P5CR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P5CR2 CRISPR/Cas9 KO Plasmid (h) | sc-406048 | 20 µg | $397.00 |
Overview
PYCR2 encodes pyrroline-5-carboxylate reductase 2 (P5CR2), a mitochondrial enzyme that catalyzes the NAD(P)H-dependent reduction of pyrroline-5-carboxylate to proline, supporting proline biosynthesis and cellular redox balance. This activity links amino acid metabolism to mitochondrial function, oxidative stress responses, and maintenance of proteostasis via proline-dependent cycling and NADP+/NADPH homeostasis. Disruption of PYCR2 has been associated with neurodevelopmental phenotypes and mitochondrial-related cellular dysfunction, making it relevant for studies of metabolic control in the nervous system. PYCR2 is therefore frequently investigated in pathways connecting mitochondrial metabolism, stress adaptation, and growth regulation.
P5CR2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PYCR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PYCR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PYCR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P5CR2 protein expression.
This CRISPR knockout system enables efficient generation of PYCR2-deficient cell models for investigation of P5CR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PYCR2 exon(s) critical for P5CR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PYCR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P5CR2 CRISPR/Cas9 KO Plasmid (h) and P5CR2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PYCR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P5CR2 HDR Plasmid (h) and P5CR2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PYCR2 homology arms to support homology-directed repair at defined PYCR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.