Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p57 Kip2 CRISPR/Cas9 KO Plasmid (h): sc-400444
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p57 Kip2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the p57 Kip2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p57 Kip2 Antibody (KP39): sc-56341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p57 Kip2 CRISPR/Cas9 KO Plasmid (h) | sc-400444 | 20 µg | $397.00 |
Overview
CDKN1C encodes p57 Kip2, a cyclin-dependent kinase inhibitor of the CIP/KIP family that restrains CDK activity to enforce G1 cell-cycle arrest and support terminal differentiation. p57 Kip2 integrates developmental and growth-control cues through regulation of Cyclin–CDK complexes and links to checkpoints that coordinate proliferation with lineage specification. The gene is imprinted and dosage-sensitive, making its expression tightly regulated in embryogenesis, placental biology, and tissue homeostasis. Dysregulation of CDKN1C is associated with imprinting-related growth disorders and has been investigated in contexts where altered cell-cycle control and differentiation contribute to disease-relevant phenotypes.
p57 Kip2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDKN1C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDKN1C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDKN1C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish p57 Kip2 protein expression.
This CRISPR knockout system enables efficient generation of CDKN1C-deficient cell models for investigation of p57 Kip2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDKN1C exon(s) critical for p57 Kip2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDKN1C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by p57 Kip2 CRISPR/Cas9 KO Plasmid (h) and p57 Kip2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDKN1C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by p57 Kip2 HDR Plasmid (h) and p57 Kip2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDKN1C homology arms to support homology-directed repair at defined CDKN1C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.