p53 Antibody (Pab 246) recommended for detection of p53 of mouse and rat origin by WB, IP and IF

p53 Antibody (Pab 246): sc-100

p53 Antibody (Pab 246) is rated 4.5 out of 5 by 8.
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Datasheets

    • Anti-p53 Antibody (Pab 246) is a mouse monoclonal IgG1 (kappa light chain) p53 antibody provided at 200 µg/ml
    • epitope mapping at amino acid residues 88-93 of mouse p53
    • Anti-p53 Antibody (Pab 246) is recommended for detection of p53 of mouse and rat origin by WB, IP and IF; may cross-react with wild type p53; non cross-reactive with mutant p53 under non-denaturing conditions
    • Anti-p53 Antibody (Pab 246) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p53 (Pab 246): sc-100.

Can I use this ab for ChIP?

Asked by: Nblanco
Thank you for your question. Yes, this p53 (Pab 246) antibody, sc-100, is recommended and warrantied for IP applications, which includes ChIP.
Answered by: Tech Service
Date published: 2020-01-06

I am going to do a Western blot experiment using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p53 (Pab 246): sc-100?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 4 out of 5 by from good Works well in western blot for mouse embryonic fibroblast
Date published: 2019-01-28
Rated 5 out of 5 by from Very good p53 antibody worked well in the mouse samples, the band is sharp and strong.
Date published: 2017-05-19
Rated 5 out of 5 by from Good p53 antibody for wild type rodent Pab 246 clone is a good choice for detection of wild type p53 in mouse and rat
Date published: 2016-12-23
Rated 5 out of 5 by from good results I'm happy to bought the product, it worked very well and showed good results
Date published: 2016-11-24
Rated 4 out of 5 by from Great data in rat placenta samples Great data in rat placenta samples, publishable images. -SCBT Publication Review
Date published: 2015-03-16
Rated 4 out of 5 by from WB: Publishable data in human bronchial smooth WB: Publishable data in human bronchial smooth muscle cells. -SCBT Publication Review
Date published: 2015-02-06
Rated 5 out of 5 by from Great antibody for mouse IP Great antibody for mouse IP, published using Swiss 3T3 cells. -SCBT Publication Review
Date published: 2015-01-15
Rated 4 out of 5 by from Works great in WB for mouse Lac Z Works great in WB for mouse Lac Z! -SCBT QC
Date published: 2014-03-12
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