p53 Antibody (Pab 246): sc-100
- p53 Antibody (Pab 246) is a mouse monoclonal IgG1 κ p53 antibody, cited in 106 publications, provided at 200 µg/ml
- epitope mapping at amino acid residues 88-93 of mouse p53
- p53 Antibody (Pab 246) is recommended for detection of p53 of mouse and rat origin by WB, IP and IF; may cross-react with wild type p53; non cross-reactive with mutant p53 under non-denaturing conditions
- Anti-p53 Antibody (Pab 246) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for p53 Antibody (Pab 246) for WB applications. This reagent is now offered in a bundle with p53 Antibody (Pab 246) (see ordering information below).
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p53 Antibody (Pab 246) is an IgG1 kappa mouse monoclonal p53 antibody (also designated TP53 antibody) that detects p53 of mouse and rat origin by WB, IP and IF. p53 Antibody (Pab 246) is available as the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p53 Antibody (Pab 246) References:
- Tumor suppressor p53 protein is a new target for the metastasis-associated Mts1/S100A4 protein: functional consequences of their interaction. | Grigorian, M., et al. 2001. J Biol Chem. 276: 22699-708. PMID: 11278647
- P53 licensed to kill? Operating the assassin. | Haupt, S., et al. 2003. J Cell Biochem. 88: 76-82. PMID: 12461776
- p53: the ultimate tumor suppressor gene? | Oren, M. 1992. FASEB J. 6: 3169-76. PMID: 1397838
- The p53-MDM2 network: from oscillations to apoptosis. | Bose, I. and Ghosh, B. 2007. J Biosci. 32: 991-7. PMID: 17914240
- Protein synthesis required to anchor a mutant p53 protein which is temperature-sensitive for nuclear transport. | Gannon, JV. and Lane, DP. 1991. Nature. 349: 802-6. PMID: 2000149
- Control of Nucleotide Metabolism Enables Mutant p53's Oncogenic Gain-of-Function Activity. | Schmidt, V., et al. 2017. Int J Mol Sci. 18: PMID: 29257071
- [The expression of p53 protein and its clinicopathological features and prognosis of esophageal spindle cell carcinoma]. | Rong, LL., et al. 2020. Zhonghua Bing Li Xue Za Zhi. 49: 715-720. PMID: 32610384
- Low-frequency collective motion of DNA-binding domain defines p53 function. | Zhang, G., et al. 2022. Proteins. 90: 881-888. PMID: 34792219
- Determination of Both TP53 Mutation Status and the Amount of p53 Protein has Limited Diagnostic Importance for Patients with Ovarian Cancer. | Klein, SM., et al. 2022. Curr Med Chem. 29: 4293-4300. PMID: 35023452
- VprBP/DCAF1 regulates p53 function and stability through site-specific phosphorylation. | Ghate, NB., et al. 2023. Oncogene. 42: 1405-1416. PMID: 37041410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p53 Antibody (Pab 246) | sc-100 | 200 µg/ml | $316.00 | |||
p53 Antibody (Pab 246): m-IgG Fc BP-HRP Bundle | sc-525418 | 200 µg Ab; 10 µg BP | $354.00 | |||
p53 Antibody (Pab 246) AC | sc-100 AC | 500 µg/ml, 25% agarose | $416.00 | |||
p53 Antibody (Pab 246) HRP | sc-100 HRP | 200 µg/ml | $316.00 | |||
p53 Antibody (Pab 246) FITC | sc-100 FITC | 200 µg/ml | $330.00 | |||
p53 Antibody (Pab 246) PE | sc-100 PE | 200 µg/ml | $343.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 488 | sc-100 AF488 | 200 µg/ml | $357.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 546 | sc-100 AF546 | 200 µg/ml | $357.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 594 | sc-100 AF594 | 200 µg/ml | $357.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 647 | sc-100 AF647 | 200 µg/ml | $357.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 680 | sc-100 AF680 | 200 µg/ml | $357.00 | |||
p53 Antibody (Pab 246) Alexa Fluor® 790 | sc-100 AF790 | 200 µg/ml | $357.00 |