p53 Antibody (Pab 240): sc-99

p53 Antibody (Pab 240) is an IgG1 kappa mouse monoclonal p53 antibody (also designated p53 antibody or TP53 antibody) that detects only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions of mouse, rat and human origin by WB, IP and IF. p53 Antibody (Pab 240) is available as the non-conjugated anti-p53 antibody form, as well as multiple conjugated forms of anti-p53 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (Pab 240) References:

1. The p53 circuit board.  |  Sullivan, KD., et al. 2012. Biochim Biophys Acta. 1825: 229-44. PMID: 22333261
2. Detecting p53 isoforms at protein level.  |  Marcel, V., et al. 2013. Methods Mol Biol. 962: 15-29. PMID: 23150434
3. p53 protein subcellular localization and apoptosis in rodent corneal epithelium cell culture following ultraviolet irradiation.  |  Tendler, Y., et al. 2013. Int J Mol Med. 31: 540-6. PMID: 23338225
4. PHD3 Regulates p53 Protein Stability by Hydroxylating Proline 359.  |  Rodriguez, J., et al. 2018. Cell Rep. 24: 1316-1329. PMID: 30067985
5. Efficient intracellular delivery of p53 protein by engineered protein crystals restores tumor suppressing function in vivo.  |  Yang, Z., et al. 2021. Biomaterials. 271: 120759. PMID: 33798968
6. Targeting the P53 Protein for Cancer Therapies: The Translational Impact of P53 Research.  |  Levine, AJ. 2022. Cancer Res. 82: 362-364. PMID: 35110395
7. Clinical significance of p53 protein expression and TP53 variation status in colorectal cancer.  |  Kim, KM., et al. 2022. BMC Cancer. 22: 940. PMID: 36045334
8. Elucidating the Implications of Diverse Dynamical Responses in p53 Protein.  |  Charan, K., et al. 2023. Chemphyschem. 24: e202200537. PMID: 36208026
9. Small-molecule correctors and stabilizers to target p53.  |  Fallatah, MMJ., et al. 2023. Trends Pharmacol Sci. 44: 274-289. PMID: 36964053
10. Posttranslational regulation of p53 tumor suppressor protein function.  |  Maxwell, SA. and Roth, JA. 1994. Crit Rev Oncog. 5: 23-57. PMID: 7948107
11. Structural aspects of the p53 protein in relation to gene evolution: a second look.  |  Soussi, T. and May, P. 1996. J Mol Biol. 260: 623-37. PMID: 8709143
12. Loss of function and p53 protein stabilization.  |  Blagosklonny, MV. 1997. Oncogene. 15: 1889-93. PMID: 9365234

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (Pab 240) has 474 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (Pab 240) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (Pab 240) Specifications

Alternate Product Name: TP53

Product Clone Name: Pab 240

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 213-217

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: only mutant p53 under non-denaturing conditions but is equally reactive with mutant and wildtype p53 under denaturing conditions

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 474 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (Pab 240): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.