p53 Antibody (Pab 1801): sc-98

p53 Antibody (Pab 1801) is an IgG1 kappa mouse monoclonal p53 antibody (also designated TP53 antibody) that detects p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. p53 Antibody (Pab 1801) is available as both conjugated and non-conjugated anti-p53 antibody forms. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

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p53 Antibody (Pab 1801) References:

1. P53 regulation and function in normal cells and tumors.  |  Liu, Y. and Kulesz-Martin, M. 2000. Medicina (B Aires). 60 Suppl 2: 9-11. PMID: 11188940
2. p53 protein regulates the effects of amifostine on apoptosis, cell cycle progression, and cytoprotection.  |  Lee, EJ., et al. 2003. Br J Cancer. 88: 754-9. PMID: 12618886
3. DNA-protein crosslinks and p53 protein expression in relation to occupational exposure to formaldehyde.  |  Shaham, J., et al. 2003. Occup Environ Med. 60: 403-9. PMID: 12771391
4. [Relevance of p53 protein and its mutations for novel strategies in cancer therapy].  |  Müller, P., et al. 2004. Cas Lek Cesk. 143: 313-7. PMID: 15305766
5. Regulation of p53--insights into a complex process.  |  Boehme, KA. and Blattner, C. 2009. Crit Rev Biochem Mol Biol. 44: 367-92. PMID: 19929178
6. p53 and its mutants in tumor cell migration and invasion.  |  Muller, PA., et al. 2011. J Cell Biol. 192: 209-18. PMID: 21263025
7. Chaperoning of mutant p53 protein by wild-type p53 protein causes hypoxic tumor regression.  |  Gogna, R., et al. 2012. J Biol Chem. 287: 2907-14. PMID: 22147694
8. p53 protein isoforms: key regulators in the front line of pathogen infections?  |  Terrier, O., et al. 2013. PLoS Pathog. 9: e1003246. PMID: 23592981
9. Case Study: discovery of inhibitors of the MDM2-p53 protein-protein interaction.  |  Liu, L., et al. 2015. Methods Mol Biol. 1278: 567-85. PMID: 25859977
10. A role for bioinorganic chemistry in the reactivation of mutant p53 in cancer.  |  Miller, JJ., et al. 2022. J Biol Inorg Chem. 27: 393-403. PMID: 35488931
11. The DNA binding activity of wild type p53 is modulated by blocking its various antigenic epitopes.  |  Wolkowicz, R., et al. 1995. Oncogene. 10: 1167-74. PMID: 7535417
12. [p53].  |  Takahashi, R. 1997. Gan To Kagaku Ryoho. 24: 1381-5. PMID: 9309129

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (Pab 1801) has 412 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (Pab 1801) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (Pab 1801) Specifications

Alternate Product Name: TP53

Product Clone Name: Pab 1801

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 32-79

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: p53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 412 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (Pab 1801): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.