p53 Antibody (D-11): sc-17846

p53 Antibody (D-11) is a mouse monoclonal antibody of the IgG1 kappa chain, raised against the full-length human p53 protein (amino acids 1–393), and specifically detects p53 from human, mouse, and rat origins in applications such as western blotting, immunoprecipitation, immunofluorescence, and enzyme-linked immunosorbent assay. p53 mouse monoclonal antibody (D-11) is widely utilized in research focused on the tumor suppressor p53, an important DNA-binding protein with oligomerization and transcription activation domains, which upregulates genes associated with cell cycle arrest and apoptosis in response to cellular stress, thereby playing a critical role in programmed cell death, cell differentiation, and cell cycle control mechanisms; p53 is primarily localized in the nucleus but can be exported to the cytoplasm by its negative regulator MDM2, an E3 ubiquitin ligase that promotes p53 degradation via the proteasome pathway. Under normal conditions, p53 levels within the cell are kept low through continuous degradation mediated by the MDM2 protein, an E3 ubiquitin ligase that targets p53 for proteasomal degradation. However, when cells experience stress signals such as DNA damage, oncogene activation, hypoxia, or nucleotide depletion, p53 becomes stabilized and accumulates in the nucleus. There, p53 functions as a transcription factor, activating or repressing a wide array of genes involved in cell cycle arrest, DNA repair, senescence, and programmed cell death (apoptosis). Further, the functional activity of p53 is modulated by post-translational modifications such as phosphorylation and acetylation, and mutations within its DNA-binding domain (amino acids 110–286) can impair its DNA-binding capacity, leading to compromised tumor suppressor functions and are implicated in numerous human cancers.

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p53 Antibody (D-11) References:

1. Expression of p53 protein and prognosis in gastric carcinoma.  |  Gürel, S., et al. 1999. J Int Med Res. 27: 85-9. PMID: 10446695
2. Estradiol stabilizes p53 protein in breast cancer cell line, MCF-7.  |  Okumura, N., et al. 2002. Jpn J Cancer Res. 93: 867-73. PMID: 12716463
3. Anti-p53 antibodies and p53 protein expression in cholangiocarcinoma.  |  Limpaiboon, T., et al. 2004. Hepatogastroenterology. 51: 25-8. PMID: 15011824
4. Constitutive expression of p53 protein in enriched normal human marrow blast cell populations.  |  Rivas, CI., et al. 1992. Blood. 79: 1982-6. PMID: 1562724
5. p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia.  |  Bártková, J., et al. 1991. Int J Cancer. 49: 196-202. PMID: 1652567
6. Influence of tetramerisation on site-specific post-translational modifications of p53: comparison of human and murine p53 tumor suppressor protein.  |  Warnock, LJ., et al. 2008. Cancer Biol Ther. 7: 1481-9. PMID: 18769132
7. p53 Research: the past thirty years and the next thirty years.  |  Lane, D. and Levine, A. 2010. Cold Spring Harb Perspect Biol. 2: a000893. PMID: 20463001
8. Reciprocal influence of the p53 and the hypoxic pathways.  |  Sermeus, A. and Michiels, C. 2011. Cell Death Dis. 2: e164. PMID: 21614094
9. Structural studies on mechanisms to activate mutant p53.  |  Viadiu, H., et al. 2014. Subcell Biochem. 85: 119-32. PMID: 25201192
10. Irradiation potentiates p53 phosphorylation and p53 binding to the promoter and coding region of the TP53 gene.  |  Legartová, S., et al. 2023. Biochimie. 204: 154-168. PMID: 36167255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (D-11) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (D-11) Specifications

Alternate Product Name: TP53

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-393

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: p53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.