P4HA2 CRISPR/Cas9 KO Plasmid (h): sc-405566
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P4HA2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P4HA2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P4HA2 CRISPR/Cas9 KO Plasmid (h) | sc-405566 | 20 µg | $397.00 |
Overview
P4HA2 encodes the alpha-2 catalytic subunit of prolyl 4-hydroxylase, a key endoplasmic reticulum enzyme required for hydroxylation of proline residues in collagen and other extracellular matrix proteins. This post-translational modification promotes proper triple-helix folding and stability, linking P4HA2 activity to collagen biosynthesis, ER protein quality control, and extracellular matrix organization. Through its role in matrix deposition and remodeling, P4HA2 is frequently studied in pathways connected to cell–matrix interactions, tissue stiffness, and microenvironmental regulation of cell behavior. Dysregulated P4HA2 expression has been associated with fibrotic remodeling and tumor-associated stromal changes, making it a relevant target for mechanistic studies of matrix-driven phenotypes.
P4HA2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P4HA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P4HA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P4HA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P4HA2 protein expression.
This CRISPR knockout system enables efficient generation of P4HA2-deficient cell models for investigation of P4HA2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P4HA2 exon(s) critical for P4HA2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P4HA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P4HA2 CRISPR/Cas9 KO Plasmid (h) and P4HA2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P4HA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P4HA2 HDR Plasmid (h) and P4HA2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P4HA2 homology arms to support homology-directed repair at defined P4HA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.