P4HA1 CRISPR/Cas9 KO Plasmid (h): sc-407173
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P4HA1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P4HA1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P4HA1 CRISPR/Cas9 KO Plasmid (h) | sc-407173 | 20 µg | $397.00 |
Overview
P4HA1 encodes the catalytic alpha subunit of prolyl 4-hydroxylase, a key ER-resident enzyme that hydroxylates proline residues in procollagen to support triple-helix stability and extracellular matrix maturation. By regulating collagen biosynthesis and deposition, P4HA1 influences matrix remodeling, cell–matrix adhesion, and tissue stiffness, processes that interface with hypoxia-responsive programs and metabolic adaptation. Altered P4HA1 activity has been linked to fibrotic phenotypes and tumor microenvironment remodeling, where extracellular matrix organization can affect invasion and stromal signaling. As a result, P4HA1 is frequently studied in pathways related to collagen fibrillogenesis, ER protein processing, and matrix-driven regulation of cell behavior.
P4HA1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P4HA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P4HA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P4HA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P4HA1 protein expression.
This CRISPR knockout system enables efficient generation of P4HA1-deficient cell models for investigation of P4HA1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P4HA1 exon(s) critical for P4HA1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P4HA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P4HA1 CRISPR/Cas9 KO Plasmid (h) and P4HA1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P4HA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P4HA1 HDR Plasmid (h) and P4HA1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P4HA1 homology arms to support homology-directed repair at defined P4HA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.