NSFL1C p47 Antibody (E-7): sc-376614

NSFL1C p47 Antibody (E-7) is a mouse monoclonal IgG2a antibody that detects NSFL1C p47 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSFL1C p47, also known as p47, UBX1, UBXD10, or UBXN2C, is a 370 amino acid protein that plays a crucial role in the fragmentation and reassembly of Golgi stacks during mitosis, a process essential for proper cell division and function. NSFL1C p47 is localized to both the nucleus and the Golgi apparatus, specifically within Golgi stacks, and contains one SEP domain and one UBX domain, which are important for interactions with other proteins. NSFL1C p47 forms a ternary complex with VCP, a protein that facilitates the fusion of transport vesicles with their target membranes, and Syntaxin 5. Through this interaction, NSFL1C p47 modulates the ATPase activity of VCP, thereby influencing the dynamics of Golgi structure during cell division. NSFL1C p47 undergoes phosphorylation during mitosis, which is critical as this modification inhibits the interaction between NSFL1C p47 and the Golgi, ensuring proper Golgi stack formation and cisternal regrowth. The high sequence identity of 89% between human NSFL1C p47 and its mouse counterpart underscores the evolutionary conservation of function across species, while the existence of multiple isoforms due to alternative splicing highlights the complexity of regulatory mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NSFL1C p47 Antibody (E-7) References:

1. Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly.  |  Yuan, X., et al. 2001. J Mol Biol. 311: 255-63. PMID: 11478859
2. VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments.  |  Wang, Y., et al. 2004. J Cell Biol. 164: 973-8. PMID: 15037600
3. The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism.  |  Bruderer, RM., et al. 2004. J Biol Chem. 279: 49609-16. PMID: 15371428
4. p97/p47-Mediated biogenesis of Golgi and ER.  |  Uchiyama, K. and Kondo, H. 2005. J Biochem. 137: 115-9. PMID: 15749824
5. The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase.  |  Kano, F., et al. 2005. Genes Cells. 10: 333-44. PMID: 15773896
6. Cdc48/p97 and Shp1/p47 regulate autophagosome biogenesis in concert with ubiquitin-like Atg8.  |  Krick, R., et al. 2010. J Cell Biol. 190: 965-73. PMID: 20855502
7. Evaluating p97 inhibitor analogues for their domain selectivity and potency against the p97-p47 complex.  |  Fang, CJ., et al. 2015. ChemMedChem. 10: 52-6. PMID: 25377500
8. PINK1 Interacts with VCP/p97 and Activates PKA to Promote NSFL1C/p47 Phosphorylation and Dendritic Arborization in Neurons.  |  Wang, KZQ., et al. 2018. eNeuro. 5: PMID: 30783609
9. p97 and p47 function in membrane tethering in cooperation with FTCD during mitotic Golgi reassembly.  |  Kaneko, Y., et al. 2021. EMBO J. 40: e105853. PMID: 33555040
10. p47 is a cofactor for p97-mediated membrane fusion.  |  Kondo, H., et al. 1997. Nature. 388: 75-8. PMID: 9214505

The preferred NSFL1C p47 antibody is NSFL1C p47 Antibody (D-9): sc-365215.