p300 Double Nickase Plasmid (h): sc-400055-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p300 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p300 Double Nickase Plasmid (h) and p300 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EP300. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p300 Antibody (F-4): sc-48343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p300 Double Nickase Plasmid (h) | sc-400055-NIC | 20 µg | $410.00 | |||
p300 Double Nickase Plasmid (h2) | sc-400055-NIC-2 | 20 µg | $410.00 |
EP300 encodes p300, a pleiotropic transcriptional coactivator and histone acetyltransferase that acetylates lysines on histones and numerous transcription factors to modulate chromatin accessibility and gene expression programs. p300 integrates signals from developmental and stress-responsive pathways, including p53-, HIF-1-, NF-κB-, TGF-β/SMAD-, and nuclear receptor–dependent transcription, thereby influencing cell cycle control, differentiation, DNA damage responses, and metabolic adaptation. As a central epigenetic regulator, EP300 perturbation is linked to widespread transcriptional dysregulation observed in diverse tumor contexts and to neurodevelopmental phenotypes, reflecting its role in enhancer activity and lineage-specific gene regulation. These features make p300 a widely used node for dissecting chromatin-mediated control of transcription and signal-dependent gene regulatory networks.
p300 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EP300 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EP300. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EP300 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EP300-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.