P2Y9 CRISPR/Cas9 KO Plasmid (m): sc-429620
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P2Y9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y9 CRISPR/Cas9 KO Plasmid (m) | sc-429620 | 20 µg | $397.00 |
Overview
Lpar4 encodes the purinergic receptor P2Y9, a G protein–coupled receptor implicated in extracellular nucleotide/lipid-mediated signaling that influences cell communication, membrane-proximal second messenger cascades, and context-dependent regulation of motility and differentiation. In mouse systems, P2Y-family signaling commonly converges on pathways controlling intracellular calcium dynamics, cAMP balance, and downstream kinase networks that coordinate cytoskeletal remodeling and transcriptional responses. Lpar4/P2Y9 activity is therefore relevant to studies of immune cell trafficking, stromal–epithelial crosstalk, and tissue homeostasis. Dysregulation of GPCR-driven purinergic signaling has been associated with inflammatory and fibrotic phenotypes as well as altered growth signaling, making Lpar4 a useful node for mechanistic pathway interrogation.
P2Y9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lpar4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lpar4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lpar4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P2Y9 protein expression.
This CRISPR knockout system enables efficient generation of Lpar4-deficient cell models for investigation of P2Y9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lpar4 exon(s) critical for P2Y9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lpar4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P2Y9 CRISPR/Cas9 KO Plasmid (m) and P2Y9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lpar4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P2Y9 HDR Plasmid (m) and P2Y9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lpar4 homology arms to support homology-directed repair at defined Lpar4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.