P2Y5 CRISPR/Cas9 KO Plasmid (h): sc-405189
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P2Y5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y5 CRISPR/Cas9 KO Plasmid (h) | sc-405189 | 20 µg | $397.00 |
Overview
LPAR6 (also known as P2Y5) encodes a G protein–coupled receptor that responds to lysophosphatidic acid to regulate intracellular signaling through heterotrimeric G proteins. P2Y5 activity influences Rho/ROCK- and MAPK-linked pathways, coordinating cytoskeletal remodeling, cell–cell adhesion, and motility in a context-dependent manner. The receptor is prominently implicated in skin and hair follicle biology, where altered LPAR6 signaling is associated with congenital hair growth disorders and broader epithelial homeostasis phenotypes. In addition, LPAR6-mediated lipid signaling intersects with inflammatory and barrier-related processes, making it relevant for mechanistic studies in keratinocytes and other epithelial models.
P2Y5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LPAR6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LPAR6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LPAR6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P2Y5 protein expression.
This CRISPR knockout system enables efficient generation of LPAR6-deficient cell models for investigation of P2Y5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LPAR6 exon(s) critical for P2Y5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LPAR6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P2Y5 CRISPR/Cas9 KO Plasmid (h) and P2Y5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LPAR6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P2Y5 HDR Plasmid (h) and P2Y5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LPAR6 homology arms to support homology-directed repair at defined LPAR6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.