P2Y2 Double Nickase Plasmid (m): sc-422096-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- P2Y2 Double Nickase Plasmid (m) and P2Y2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting P2ry2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P2Y2 Antibody (H-5): sc-518121
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y2 Double Nickase Plasmid (m) | sc-422096-NIC | 20 µg | $410.00 | |||
P2Y2 Double Nickase Plasmid (m2) | sc-422096-NIC-2 | 20 µg | $410.00 |
Mouse P2ry2 encodes the P2Y2 receptor, a G protein–coupled purinergic receptor activated by extracellular ATP and UTP that couples primarily to Gq/11 to stimulate phospholipase C, IP3-dependent calcium mobilization, and PKC/MAPK signaling. P2Y2 regulates epithelial ion and fluid transport, mucociliary clearance, and mechanotransduction, and it modulates cytoskeletal remodeling, cell migration, and cytokine production in multiple tissues. Through these pathways, P2Y2 contributes to innate immune signaling and inflammatory responses, with research relevance to airway biology, vascular remodeling, and tissue injury and repair models. Dysregulated purinergic signaling involving P2Y2 has been studied in contexts of chronic inflammation and fibrosis, as well as in tumor microenvironment and metastatic processes in experimental systems.
P2Y2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the P2ry2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within P2ry2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt P2ry2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of P2ry2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.