P2Y11 CRISPR/Cas9 KO Plasmid (h): sc-405745
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2Y11 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the P2Y11 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2Y11 CRISPR/Cas9 KO Plasmid (h) | sc-405745 | 20 µg | $397.00 |
Overview
P2RY11 encodes the human P2Y11 receptor, an ATP/UTP-responsive G protein-coupled receptor that uniquely couples to both Gq and Gs signaling. Upon extracellular nucleotide stimulation, P2Y11 promotes phospholipase C activation with intracellular Ca²⁺ mobilization and elevates cAMP, integrating purinergic inputs with MAPK/ERK and other second-messenger pathways that shape immune cell activation and cytokine programs. P2Y11 is expressed in multiple leukocyte populations and is implicated in regulation of dendritic cell, monocyte, and T cell responses within inflammatory microenvironments. Dysregulated purinergic signaling involving P2Y11 has been associated with inflammatory and autoimmune phenotypes, supporting its study in immunometabolism, neuroinflammation, and tumor-immune interaction models.
P2Y11 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the P2RY11 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the P2RY11 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the P2RY11 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish P2Y11 protein expression.
This CRISPR knockout system enables efficient generation of P2RY11-deficient cell models for investigation of P2Y11 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting P2RY11 exon(s) critical for P2Y11 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple P2RY11 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by P2Y11 CRISPR/Cas9 KO Plasmid (h) and P2Y11 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the P2RY11 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by P2Y11 HDR Plasmid (h) and P2Y11 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by P2RY11 homology arms to support homology-directed repair at defined P2RY11 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.