Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
P2X4 Double Nickase Plasmid (h): sc-401779-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- P2X4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- P2X4 Double Nickase Plasmid (h) and P2X4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting P2RX4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: P2X4 Antibody (D-3): sc-518167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2X4 Double Nickase Plasmid (h) | sc-401779-NIC | 20 µg | $410.00 |
P2RX4 encodes the human P2X4 receptor, an ATP-gated, nonselective cation channel that mediates rapid Ca²⁺ and Na⁺ influx in response to extracellular nucleotides. P2X4 signaling contributes to purinergic neurotransmission, microglial activation, and inflammatory crosstalk, integrating with pathways that regulate cytokine release, chemotaxis, and cellular excitability. In immune and nervous system contexts, P2X4 activity is linked to mechanisms underlying neuroinflammation and neuropathic pain, and it has been studied in relation to vascular and metabolic stress responses. As a membrane ion channel responsive to damage-associated ATP, P2X4 is a useful node for interrogating ATP-driven signaling and ion flux-dependent transcriptional programs.
P2X4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the P2RX4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within P2RX4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt P2RX4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of P2RX4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.