P2X4 Antibody (D-3): sc-518167
- P2X4 Antibody (D-3) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 360-382 of P2X4 of human origin
- recommended for detection of P2X4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-P2X4 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for P2X4 Antibody (D-3) for WB applications. This reagent is now offered in a bundle with P2X4 Antibody (D-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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P2X4 Antibody (D-3) is a mouse monoclonal IgM antibody that detects P2X4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). P2X4 Antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. P2X4 receptor is a member of the P2X receptor family, which consists of ligand-gated ion channels that facilitate calcium ion influx into cells in response to extracellular ATP. P2X4 receptors play a crucial role in mediating synaptic transmission and are expressed in diverse tissues such as neurons, prostate, bladder, pancreas, colon, testis, and ovary. P2X4 is involved in various physiological processes, including pain perception and inflammation, making P2X4 a significant target for research in neurobiology and pharmacology. P2X4 receptor is characterized by intracellular amino- and carboxy-termini that are essential for channel function and regulation. Understanding P2X4 structural aspects is vital, as conformational alterations can influence receptor activity and cellular responses to ATP. P2X4 Antibody (D-3) serves as an invaluable tool for researchers investigating P2X4′s role in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
P2X4 Antibody (D-3) References:
- Cytolytic P2X purinoceptors. | Di Virgilio, F., et al. 1998. Cell Death Differ. 5: 191-9. PMID: 10200464
- Allosteric modulation and accelerated resensitization of human P2X(3) receptors by cibacron blue. | Alexander, K., et al. 1999. J Pharmacol Exp Ther. 291: 1135-42. PMID: 10565834
- Inactivation of P2X2 purinoceptors by divalent cations. | Ding, S. and Sachs, F. 2000. J Physiol. 522 Pt 2: 199-214. PMID: 10639098
- Evidence for P2X(3), P2X(4), P2X(5) but not for P2X(7) containing purinergic receptors in Müller cells of the rat retina. | Jabs, R., et al. 2000. Brain Res Mol Brain Res. 76: 205-10. PMID: 10762695
- A natural dominant negative P2X1 receptor due to deletion of a single amino acid residue. | Oury, C., et al. 2000. J Biol Chem. 275: 22611-4. PMID: 10816552
- P2X receptors in sensory neurones. | Burnstock, G. 2000. Br J Anaesth. 84: 476-88. PMID: 10823099
- Pharmacology of cloned P2X receptors. | North, RA. and Surprenant, A. 2000. Annu Rev Pharmacol Toxicol. 40: 563-80. PMID: 10836147
- P2X4 Receptor Function in the Nervous System and Current Breakthroughs in Pharmacology. | Stokes, L., et al. 2017. Front Pharmacol. 8: 291. PMID: 28588493
- P2X4 and lysosome fusion. | Murrell-Lagnado, RD. and Frick, M. 2019. Curr Opin Pharmacol. 47: 126-132. PMID: 31039505
- Crosstalking interactions between P2X4 and 5-HT3A receptors. | Chang-Halabi, Y., et al. 2023. Neuropharmacology. 236: 109574. PMID: 37156336
- Methods for studying P2X4 receptor ion channels in immune cells. | Sluyter, R., et al. 2024. J Immunol Methods. 526: 113626. PMID: 38311008
- The human P2x1 receptor: molecular cloning, tissue distribution, and localization to chromosome 17. | Longhurst, PA., et al. 1996. Biochim Biophys Acta. 1308: 185-8. PMID: 8809107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2X4 Antibody (D-3) | sc-518167 | 200 µg/ml | $322.00 | |||
P2X4 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-550683 | 200 µg Ab; 40 µg BP | $361.00 | |||
P2X4 Antibody (D-3) AC | sc-518167 AC | 500 µg/ml, 25% agarose | $424.00 | |||
P2X4 Antibody (D-3) HRP | sc-518167 HRP | 200 µg/ml | $322.00 | |||
P2X4 Antibody (D-3) FITC | sc-518167 FITC | 200 µg/ml | $336.00 | |||
P2X4 Antibody (D-3) PE | sc-518167 PE | 200 µg/ml | $349.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 488 | sc-518167 AF488 | 200 µg/ml | $364.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 546 | sc-518167 AF546 | 200 µg/ml | $364.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 594 | sc-518167 AF594 | 200 µg/ml | $364.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 647 | sc-518167 AF647 | 200 µg/ml | $364.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 680 | sc-518167 AF680 | 200 µg/ml | $364.00 | |||
P2X4 Antibody (D-3) Alexa Fluor® 790 | sc-518167 AF790 | 200 µg/ml | $364.00 |