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CDKN1B/Kip1 p27 Antibody (0.N.491) - Western Blotting - Image 349954
p27 Kip1 Antibody (0.N.491): sc-71813. Western blot analysis of p27 Kip1 expression in MDA-MB-231 (A), MM-142 (B), NIH/3T3 (C) and SK-BR-3 (D) whole cell lysates and human breast tissue extract (E).
p27 Kip1 Antibody (0.N.491): sc-71813. Western blot analysis of p27 Kip1 expression in MDA-MB-231 (A), MM-142 (B), NIH/3T3 (C) and SK-BR-3 (D) whole cell lysates and human breast tissue extract (E).

CDKN1B/Kip1 p27 Antibody (0.N.491): sc-71813

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Datasheets
  • CDKN1B/Kip1 p27 Antibody (0.N.491) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
  • raised against full length p27 Kip1 of mouse origin
  • recommended for detection of p27 Kip1 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with other mitotic inhibitors
  • See CDKN1B/Kip1 p27 (F-8): sc-1641 for CDKN1B/Kip1 p27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CDKN1B/Kip1 p27 Antibody (0.N.491) for WB and IHC(P) applications. These reagents are now offered in bundles with CDKN1B/Kip1 p27 Antibody (0.N.491) (see ordering information below).
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SEE ALSO...

p27 Kip1 Antibody (0.N.491) is a mouse monoclonal IgG1 antibody that detects p27 Kip1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p27 Kip1 plays a crucial role as a negative regulator of cell cycle progression, particularly during the G1 phase, where p27 Kip1 inhibits the activity of cyclin-dependent kinases (Cdks) by binding to D-type cyclins and Cdk4, thereby preventing the transition to the S phase. This regulation is vital for maintaining proper cell growth and division, especially in response to external signals such as TGFβ, which can induce G1 arrest. p27 Kip1′s ability to modulate cell cycle progression highlights p27 Kip1′s importance in tumor suppression, as dysregulation can lead to uncontrolled cell proliferation and cancer development. Anti-p27 Kip1 antibody (0.N.491) serves as an essential tool for researchers studying cell cycle dynamics and the molecular mechanisms underlying various diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

CDKN1B/Kip1 p27 Antibody (0.N.491) References:

  1. p27(Kip1) controls cytokinesis via the regulation of citron kinase activation.  |  Serres, MP., et al. 2012. J Clin Invest. 122: 844-58. PMID: 22293177
  2. p27(KIP1) and PTEN cooperate in myeloproliferative neoplasm tumor suppression in mice.  |  Shao, J., et al. 2015. Exp Hematol Oncol. 5: 17. PMID: 27366593
  3. Loss of p27kip1 increases genomic instability and induces radio-resistance in luminal breast cancer cells.  |  Berton, S., et al. 2017. Sci Rep. 7: 595. PMID: 28377607
  4. P21Waf1/Cip1 and p27Kip1 are correlated with the development and invasion of prolactinoma.  |  Dong, W., et al. 2018. J Neurooncol. 136: 485-494. PMID: 29230669
  5. Clinical Impact of p27Kip1 and CaSR Expression on Primary Hyperparathyroidism.  |  Sengul Aycicek, G., et al. 2018. Endocr Pathol. 29: 250-258. PMID: 29589297
  6. Akt/p27kip1 Pathway Is Not Involved in Human Insulinoma Tumorigenesis.  |  Díaz, AG., et al. 2018. Int J Endocrinol. 2018: 7865072. PMID: 29853883
  7. The AMPK/p27Kip1 Axis Regulates Autophagy/Apoptosis Decisions in Aged Skeletal Muscle Stem Cells.  |  White, JP., et al. 2018. Stem Cell Reports. 11: 425-439. PMID: 30033086
  8. Stabilization of p27Kip1/CDKN1B by UBCH7/UBE2L3 catalyzed ubiquitinylation: a new paradigm in cell-cycle control.  |  Whitcomb, EA., et al. 2019. FASEB J. 33: 1235-1247. PMID: 30113882
  9. Loss of p27kip1 suppresses the myocardial senescence caused by estrogen deficiency.  |  Yan, Y., et al. 2019. J Cell Biochem. 120: 13994-14003. PMID: 30957908
  10. A novel CDK-independent function of p27Kip1 in preciliary vesicle trafficking during ciliogenesis.  |  Yukimoto, H., et al. 2020. Biochem Biophys Res Commun. 527: 716-722. PMID: 32423824

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CDKN1B/Kip1 p27 Antibody (0.N.491)

sc-71813
200 µg/ml
$322.00

CDKN1B/Kip1 p27 Antibody (0.N.491): m-IgG Fc BP-HRP Bundle

sc-539413
200 µg Ab; 10 µg BP
$361.00

CDKN1B/Kip1 p27 Antibody (0.N.491): m-IgG1 BP-HRP Bundle

sc-541477
200 µg Ab; 20 µg BP
$361.00

Can p27 (0.N.491): sc-71813 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, p27 (0.N.491): sc-71813 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by Xiong Lab from Good for detecting mouse and human p27 by WBGood for detecting p27 in mouse (MEF) and human (U2OS) cells at 1:500 dilution.
Date published: 2018-11-14
Rated 5 out of 5 by ANNIE2 from It is useful for Western blot!I tested this antibody for TPA or DMBA experiments. While the cells occur apoptosis, this antibody worked, showed its activation.
Date published: 2018-07-05
Rated 5 out of 5 by UNC123 from Great antibody! worked in my hand.A single strong band was observed using this antibody in various WCLs by western. The signals are always strong and clear.
Date published: 2017-09-11
Rated 5 out of 5 by caiqinren from good jobI used it at a dilution of 1:1000 for WB incubated in 4℃ overnight, and the results were quite good! Clean band with little background for WB in mcf7 and 293t whole cell lysates!
Date published: 2016-12-30
Rated 5 out of 5 by skhou from Excellent antibodiyA good antibody!!!This antibody works very well in western blot detection in the H1299,hela,u2os cells.
Date published: 2016-12-27
Rated 5 out of 5 by 七天大圣 from Western blot of this antibodyi use this antibody to select high expression p27 celline,at last i got the different exprsssion levels band,very useful!
Date published: 2016-12-13
Rated 5 out of 5 by jjhou from strong signal!I used it at a dilution of 1:500 for WB, and the results were great!Clean band with little background for WB in h1299, sw620, hela, u2os whole cell lysates!!
Date published: 2016-12-07
Rated 4 out of 5 by MS from CleanClean, singular band observed in western blot analysis of Jurkat and human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-25
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CDKN1B/Kip1 p27 Antibody (0.N.491) is rated 4.6 out of 5 by 11.
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