p22-phox Antibody (E-11): sc-271968

p22-phox Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize p22-phox protein, also known as CYBA or cytochrome b-245 alpha chain, from human origin. anti-p22-phox antibody (E-11) targets an epitope spanning amino acids 133-159 near the C-terminus of full-length p22-phox, facilitating detection in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p22-phox is a pivotal component of the NADPH oxidase complex, which is essential for the generation of superoxide radicals in various cell types, including phagocytes, neuroepithelial cells, vascular smooth muscle cells, and endothelial cells. Structurally, p22-phox pairs with gp91-phox (NOX2) to form the membrane-bound flavocytochrome b558, a critical element for electron transfer and reactive oxygen species production. Post-translational modifications of p22-phox, such as phosphorylation, are vital for the regulation and activation of the NADPH oxidase complex, enabling dynamic interactions with cytosolic proteins like p47-phox and p67-phox during enzyme activation. These interactions are essential for assembling the active enzyme complex at the plasma membrane, which is necessary for effective immune responses and cellular signaling. Additionally, p22-phox functions as a surface oxygen sensor, initiating signaling pathways in response to hypoxic conditions, thereby playing a key role in cellular adaptation and survival. p22-phox (E-11) antibody is available in both non-conjugated form and various conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, offering versatile options for a wide range of experimental applications. p22-phox monoclonal antibody (E-11) is an indispensable tool for researchers investigating oxidative stress, immune responses, and the intricate mechanisms of cellular signaling, enabling comprehensive studies of the structural and functional dynamics of the NADPH oxidase complex.

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p22-phox Antibody (E-11) References:

1. O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase.  |  Archer, SL., et al. 1999. Proc Natl Acad Sci U S A. 96: 7944-9. PMID: 10393927
2. The p67(phox) activation domain regulates electron flow from NADPH to flavin in flavocytochrome b(558).  |  Nisimoto, Y., et al. 1999. J Biol Chem. 274: 22999-3005. PMID: 10438466
3. Cell transformation by the superoxide-generating oxidase Mox1.  |  Suh, YA., et al. 1999. Nature. 401: 79-82. PMID: 10485709
4. Superoxide generation in transformed B-lymphocytes from patients with severe, malignant osteopetrosis.  |  Yang, S., et al. 1999. Mol Cell Biochem. 199: 15-24. PMID: 10544947
5. Identification of a functional leukocyte-type NADPH oxidase in human endothelial cells :a potential atherogenic source of reactive oxygen species.  |  Meyer, JW., et al. 1999. Endothelium. 7: 11-22. PMID: 10599557
6. Monocyte NADPH oxidase subunit p22(phox) and inducible hemeoxygenase-1 gene expressions are increased in type II diabetic patients: relationship with oxidative stress.  |  Avogaro, A., et al. 2003. J Clin Endocrinol Metab. 88: 1753-9. PMID: 12679469
7. Preliminary characterisation of the promoter of the human p22(phox) gene: identification of a new polymorphism associated with hypertension.  |  Moreno, MU., et al. 2003. FEBS Lett. 542: 27-31. PMID: 12729892
8. Molecular basis of phosphorylation-induced activation of the NADPH oxidase.  |  Groemping, Y., et al. 2003. Cell. 113: 343-55. PMID: 12732142
9. Increased myocardial NADPH oxidase activity in human heart failure.  |  Heymes, C., et al. 2003. J Am Coll Cardiol. 41: 2164-71. PMID: 12821241
10. The arachidonate-activable, NADPH oxidase-associated H+ channel. Evidence that gp91-phox functions as an essential part of the channel.  |  Henderson, LM., et al. 1995. J Biol Chem. 270: 5909-16. PMID: 7890722
11. p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells.  |  Ushio-Fukai, M., et al. 1996. J Biol Chem. 271: 23317-21. PMID: 8798532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p22-phox Antibody (E-11) has 51 citations in a variety of scientific publications.

Primary antibodies like Anti-p22-phox Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p22-phox Antibody (E-11) Specifications

Alternate Product Name: CYBA

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 133-159

Epitope Target: p22-phox

Epitope Species: human

Product Reactivity/Detection: p22-phox

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 51 citations in various scientific publications

Available Conjugates/Forms for p22-phox Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin