Waf1/Cip1/CDKN1A p21 (m): 293T Lysate: sc-122305

p21 Waf1/Cip1 (m): 293T Lysate is derived from the human embryonic kidney 293T cell line genetically modified to express the mouse isoform of the cyclin-dependent kinase inhibitor p21 Waf1/Cip1. p21 is a crucial regulator of cell cycle progression and is involved in mediating cell cycle arrest in response to various cellular stresses, including DNA damage and oncogenic signaling. The 293T cell line is commonly utilized for producing lysates due to its high transfection efficiency and robust protein expression capabilities, making it suitable for generating p21 Waf1/Cip1 lysates. In scientific research, p21 Waf1/Cip1 (m): 293T Lysate serves as a valuable tool for investigating the molecular mechanisms underlying cell cycle regulation and tumor suppression. Researchers utilize this lysate in biochemical assays such as Western blotting, immunoprecipitation, and kinase assays to analyze the expression levels of p21 and its interactions with cyclin-dependent kinases (CDKs) and cyclins. Moreover, p21 Waf1/Cip1 (m): 293T Lysate is employed in functional studies to explain the role of p21 in cellular processes such as cell proliferation, senescence, and apoptosis. By utilizing this lysate, researchers can gain insights into the complex regulatory networks governing cell cycle progression and the potential implications of p21 dysregulation in cancer development and progression.