CDKN2A/p19ARF Antibody (5-C3-1): sc-32748

p19 ARF Antibody (5-C3-1) is a rat monoclonal IgG2b antibody that detects the p19 ARF protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p19 ARF Antibody (5-C3-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p19 ARF plays a crucial role in regulating the cell cycle by acting as a tumor suppressor, particularly through interaction with the p53 pathway. p19 ARF is essential for maintaining genomic stability and preventing uncontrolled cell proliferation, which can lead to tumorigenesis. Notably, p19 ARF is derived from an alternative reading frame of the p16 gene, and while distinct from p16, shares the ability to induce cell cycle arrest. Studies show that mice lacking p19 ARF, despite having functional p16, develop tumors at an early age, highlighting p19 ARF′s critical role in cancer prevention. Disruptions in p19 ARF expression have been implicated in a significant percentage of human T cell acute lymphoblastic leukemias, making p19 ARF a vital target for research in cancer biology and therapeutic development.

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CDKN2A/p19ARF Antibody (5-C3-1) References:

1. Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.  |  Bertwistle, D., et al. 2004. Hybrid Hybridomics. 23: 293-300. PMID: 15672607
2. p19(Arf) is required for the cellular response to chronic DNA damage.  |  Bieging-Rolett, KT., et al. 2016. Oncogene. 35: 4414-21. PMID: 26725325
3. p19(Arf) limits primary vitreous cell proliferation driven by PDGF-B.  |  Iqbal, NS., et al. 2016. Exp Eye Res. 145: 224-229. PMID: 26778750
4. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction.  |  Mikawa, R., et al. 2020. Biomolecules. 10: PMID: 32192082
5. A novel p16INK4A transcript.  |  Mao, L., et al. 1995. Cancer Res. 55: 2995-7. PMID: 7541708
6. A cell cycle regulator potentially involved in genesis of many tumor types.  |  Kamb, A., et al. 1994. Science. 264: 436-40. PMID: 8153634
7. Braking the cycle.  |  Hunter, T. 1993. Cell. 75: 839-41. PMID: 8252620
8. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4.  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
9. Mammalian G1 cyclins.  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492
10. Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest.  |  Quelle, DE., et al. 1995. Cell. 83: 993-1000. PMID: 8521522
11. Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF.  |  Kamijo, T., et al. 1997. Cell. 91: 649-59. PMID: 9393858
12. Genomic alterations of the p19ARF encoding exons in T-cell acute lymphoblastic leukemia.  |  Gardie, B., et al. 1998. Blood. 91: 1016-20. PMID: 9446664

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CDKN2A/p19ARF Antibody (5-C3-1) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-CDKN2A/p19ARF Antibody (5-C3-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CDKN2A/p19ARF Antibody (5-C3-1) Specifications

Alternate Product Name: p19ARF (mouse) tumor suppressor

Product Clone Name: 5-C3-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: p19 ARF

Epitope Species: mouse

Product Reactivity/Detection: p19 ARF

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for CDKN2A/p19ARF Antibody (5-C3-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin