p16-ARC Antibody (C-3): sc-166760
- p16-ARC Antibody (C-3) is a mouse monoclonal IgG2b κ p16-ARC antibody, cited in 22 publications, provided at 200 µg/ml
- raised against amino acids 1-151 representing full length p16-ARC of human origin
- p16-ARC Antibody (C-3) is recommended for detection of p16-ARC of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-p16-ARC Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p16-ARC Antibody (C-3) for WB applications. These reagents are now offered in bundles with p16-ARC Antibody (C-3) (see ordering information below).
p16-ARC Antibody (C-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects p16-ARC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p16-ARC (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p16-ARC protein, also known as ARPC5 or ARC16, is a crucial 151 amino acid subunit of the Arp2/3 complex, which is essential for actin polymerization and the formation of branched actin networks. p16-ARC protein plays a significant role in maintaining the structural integrity of the Arp2/3 complex, and serves as a substrate for MAPKAPK-2, which phosphorylates p16-ARC, potentially influencing the regulatory functions of the Arp2/3 complex and the remodeling of the actin cytoskeleton. p16-ARC′s interaction with other actin-related proteins underscores its importance in cellular processes such as motility, shape maintenance, and intracellular transport. Two isoforms of p16-ARC arise from alternative splicing, which may contribute to functional diversity in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p16-ARC Antibody (C-3) References:
- Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. | Zhao, X., et al. 2001. Biochem Biophys Res Commun. 280: 513-7. PMID: 11162547
- Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity. | Gournier, H., et al. 2001. Mol Cell. 8: 1041-52. PMID: 11741539
- Characterization of Arp2/3 complex in chicken tissues. | Terasaki, AG., et al. 2002. Cell Struct Funct. 27: 383-91. PMID: 12502893
- Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis. | Singh, S., et al. 2003. J Biol Chem. 278: 36410-7. PMID: 12829704
- Pathway of actin filament branch formation by Arp2/3 complex. | Beltzner, CC. and Pollard, TD. 2008. J Biol Chem. 283: 7135-44. PMID: 18165685
- The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. | Welch, MD., et al. 1997. J Cell Biol. 138: 375-84. PMID: 9230079
- Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. | Machesky, LM., et al. 1997. Biochem J. 328 (Pt 1): 105-12. PMID: 9359840
- Identification and characterisation of a novel human isoform of Arp2/3 complex subunit p16-ARC/ARPC5. | Millard, TH., et al. 2003. Cell Motil Cytoskeleton. 54: 81-90. PMID: 12451597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p16-ARC Antibody (C-3) | sc-166760 | 200 µg/ml | $322.00 | |||
p16-ARC Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-521734 | 200 µg Ab, 40 µg BP | $361.00 | |||
p16-ARC Antibody (C-3): m-IgG2b BP-HRP Bundle | sc-548962 | 200 µg Ab; 10 µg BP | $361.00 | |||
p16-ARC Antibody (C-3) AC | sc-166760 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p16-ARC Antibody (C-3) HRP | sc-166760 HRP | 200 µg/ml | $322.00 | |||
p16-ARC Antibody (C-3) FITC | sc-166760 FITC | 200 µg/ml | $336.00 | |||
p16-ARC Antibody (C-3) PE | sc-166760 PE | 200 µg/ml | $349.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 488 | sc-166760 AF488 | 200 µg/ml | $364.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 546 | sc-166760 AF546 | 200 µg/ml | $364.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 594 | sc-166760 AF594 | 200 µg/ml | $364.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 647 | sc-166760 AF647 | 200 µg/ml | $364.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 680 | sc-166760 AF680 | 200 µg/ml | $364.00 | |||
p16-ARC Antibody (C-3) Alexa Fluor® 790 | sc-166760 AF790 | 200 µg/ml | $364.00 |