CDKN2A/p16 Antibody (F-12): sc-1661

p16 INK4A Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects p16 INK4A of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). p16 INK4A monoclonal antibody (F-12) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. p16 INK4A (F-12) antibody targets a protein that functions as a negative regulator of the cell cycle, specifically inhibiting cyclin-dependent kinase 4 (Cdk4) activity, which is essential for the transition from G1 phase to S phase of the cell cycle. By binding to Cdk4, p16 INK4A prevents formation of the active Cdk4/cyclin D complex, thereby halting cell proliferation and maintaining cellular integrity. This regulatory function is particularly important in tumor suppression, as p16 INK4A is frequently found to be mutated or deleted in various human cancers, highlighting significance in cancer biology and potential for therapeutic targeting.

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CDKN2A/p16 Antibody (F-12) References:

1. The physiology of p16(INK4A)-mediated G1 proliferative arrest.  |  Shapiro, GI., et al. 2000. Cell Biochem Biophys. 33: 189-97. PMID: 11325039
2. Implications of Genetic and Epigenetic Alterations of CDKN2A (p16(INK4a)) in Cancer.  |  Zhao, R., et al. 2016. EBioMedicine. 8: 30-39. PMID: 27428416
3. Therapeutic Strategies for Targeting CDKN2A Loss in Melanoma.  |  Kreuger, IZM., et al. 2023. J Invest Dermatol. 143: 18-25.e1. PMID: 36123181
4. The SMAD2/miR-4256/HDAC5/p16INK4a signaling axis contributes to gastric cancer progression.  |  Wang, M., et al. 2023. Oncol Res. 31: 515-541. PMID: 37415735
5. A cell cycle regulator potentially involved in genesis of many tumor types.  |  Kamb, A., et al. 1994. Science. 264: 436-40. PMID: 8153634
6. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.  |  Harper, JW., et al. 1993. Cell. 75: 805-16. PMID: 8242751
7. WAF1, a potential mediator of p53 tumor suppression.  |  el-Deiry, WS., et al. 1993. Cell. 75: 817-25. PMID: 8242752
8. Braking the cycle.  |  Hunter, T. 1993. Cell. 75: 839-41. PMID: 8252620
9. p21 is a universal inhibitor of cyclin kinases.  |  Xiong, Y., et al. 1993. Nature. 366: 701-4. PMID: 8259214
10. A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4.  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
11. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.  |  Polyak, K., et al. 1994. Genes Dev. 8: 9-22. PMID: 8288131
12. Mammalian G1 cyclins.  |  Sherr, CJ. 1993. Cell. 73: 1059-65. PMID: 8513492

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CDKN2A/p16 Antibody (F-12) has 530 citations in a variety of scientific publications.

Primary antibodies like Anti-CDKN2A/p16 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CDKN2A/p16 Antibody (F-12) Specifications

Alternate Product Name: CDKN2A/p16 INK4A

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-168

Epitope Target: p16 INK4A

Epitope Species: mouse

Product Reactivity/Detection: p16 INK4A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 530 citations in various scientific publications

Available Conjugates/Forms for CDKN2A/p16 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CDKN2A/p16 antibody is CDKN2A/p16 Antibody (JC8): sc-56330.