p-Thr Antibody (H-2): sc-5267

p-Thr Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphothreonine across a broad range of species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). p-Thr (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosphothreonine plays a crucial role in cellular signaling, particularly in the activation of mitogen-activated protein kinases (MAPKs) such as ERK and JNK, which are essential for regulating various cellular processes, including growth, differentiation, and response to stress. The phosphorylation of threonine residues is catalyzed by several families of protein kinases, including Raf, Rsk, ROCK, PAK, Akt, and PKC, which underscores the importance of threonine phosphorylation in modulating signaling pathways. Anti-phosphothreonine antibody (H-2) enables researchers to effectively characterize proteins with phosphorylated threonine residues and gain insights into the intricate cellular pathways that involve threonine phosphorylation, thereby advancing our understanding of cellular functions and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Thr Antibody (H-2) References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region.  |  Bellacosa, A., et al. 1991. Science. 254: 274-7. PMID: 1833819
3. Structure and function of the phosphothreonine-specific FHA domain.  |  Mahajan, A., et al. 2008. Sci Signal. 1: re12. PMID: 19109241
4. Divergent Stereoselectivity in Phosphothreonine (pThr)-Catalyzed Reductive Aminations of 3-Amidocyclohexanones.  |  Shugrue, CR., et al. 2018. J Org Chem. 83: 4491-4504. PMID: 29547285
5. Protein kinase C phosphorylation at Thr 654 of the unoccupied EGF receptor and EGF binding regulate functional receptor loss by independent mechanisms.  |  Lin, CR., et al. 1986. Cell. 44: 839-48. PMID: 3006920
6. Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner.  |  Hou, M., et al. 2019. Infect Immun. 87: PMID: 30396897
7. Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine.  |  Moen, JM., et al. 2022. Nat Commun. 13: 7226. PMID: 36433969
8. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
9. Constitutive mutant and putative regulatory serine phosphorylation site of mammalian MAP kinase kinase (MEK1).  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
10. Phosphorylation of the c-Fos transrepression domain by mitogen-activated protein kinase and 90-kDa ribosomal S6 kinase.  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
11. ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice.  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
12. Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Thr Antibody (H-2) has 94 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Thr Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Thr Antibody (H-2) Specifications

Alternate Product Name: phosphorylated threonine

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: N/A

Epitope Target: p-Thr

Epitope Species:

Product Reactivity/Detection: phosphothreonine-containing proteins

Species Reactivity/Detection: broad

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 94 citations in various scientific publications

Available Conjugates/Forms for p-Thr Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin