p-Tau Antibody (PHF-13): sc-32275
- p-Tau Antibody (PHF-13) is a mouse monoclonal IgG2b κ, cited in 25 publications, provided at 200 µg/ml
- raised against highly purified PHF-Tau preparation of human origin
- Anti-p-Tau Antibody (PHF-13) is recommended for detection of Tau phosphorylated at Ser 396 of mouse, rat and human origin by WB, IP and IF
- Anti-p-Tau Antibody (PHF-13) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Tau Antibody (PHF-13). This work is in progress.
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p-Tau Antibody (PHF-13) is a mouse monoclonal IgG2b kappa light chain antibody that detects p-Tau phosphorylated at Ser 396 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. anti-p-Tau antibody (PHF-13) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tau protein, also known as MAPT (microtubule-associated protein tau), is a 758 amino acid protein that plays a crucial role in stabilizing microtubules, essential for maintaining neuronal structure and function. p-Tau (PHF-13) monoclonal antibody promotes microtubule assembly and stability, vital for neuronal polarity and cytoskeleton integrity. Tau interacts with various proteins, including tubulin and other microtubule-associated proteins, facilitating dynamic cytoskeleton nature, important for axonal transport and synaptic function. Tau undergoes numerous post-translational modifications, including phosphorylation, influencing function and interactions, with abnormalities linked to neurodegenerative diseases like Alzheimer′s disease, pallido-ponto-nigral degeneration, corticobasal degeneration, and progressive supranuclear palsy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Tau Antibody (PHF-13) References:
- Hyperphosphorylated tau in SY5Y cells: similarities and dissimilarities to abnormally hyperphosphorylated tau from Alzheimer disease brain. | Zhong, J., et al. 1999. FEBS Lett. 453: 224-8. PMID: 10403408
- Regulation of expression, phosphorylation and biological activity of tau during differentiation in SY5Y cells. | Haque, N., et al. 1999. Brain Res. 838: 69-77. PMID: 10446318
- 14-3-3zeta is an effector of tau protein phosphorylation. | Hashiguchi, M., et al. 2000. J Biol Chem. 275: 25247-54. PMID: 10840038
- Mechanism of neurofibrillary degeneration and pharmacologic therapeutic approach. | Iqbal, K., et al. 2000. J Neural Transm Suppl. 59: 213-22. PMID: 10961432
- Phosphorylation of tau is regulated by PKN. | Taniguchi, T., et al. 2001. J Biol Chem. 276: 10025-31. PMID: 11104762
- Hyperphosphorylation induces self-assembly of tau into tangles of paired helical filaments/straight filaments. | Alonso, A., et al. 2001. Proc Natl Acad Sci U S A. 98: 6923-8. PMID: 11381127
- Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain. | Hoshi, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 2719-23. PMID: 8610107
- Differential phosphorylation of human tau isoforms containing three repeats by several protein kinases. | Singh, TJ., et al. 1996. Arch Biochem Biophys. 328: 43-50. PMID: 8638936
- Somatodendritic localization of phosphorylated tau in neonatal and adult rat cerebral cortex. | Tashiro, K., et al. 1997. Neuroreport. 8: 2797-801. PMID: 9295120
- Mechanisms of neurofibrillary degeneration and the formation of neurofibrillary tangles. | Iqbal, K., et al. 1998. J Neural Transm Suppl. 53: 169-80. PMID: 9700655
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Tau Antibody (PHF-13) | sc-32275 | 200 µg/ml | $322.00 | |||
p-Tau Antibody (PHF-13) AC | sc-32275 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-Tau Antibody (PHF-13) HRP | sc-32275 HRP | 200 µg/ml | $322.00 | |||
p-Tau Antibody (PHF-13) FITC | sc-32275 FITC | 200 µg/ml | $336.00 | |||
p-Tau Antibody (PHF-13) PE | sc-32275 PE | 200 µg/ml | $349.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 488 | sc-32275 AF488 | 200 µg/ml | $364.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 546 | sc-32275 AF546 | 200 µg/ml | $364.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 594 | sc-32275 AF594 | 200 µg/ml | $364.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 647 | sc-32275 AF647 | 200 µg/ml | $364.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 680 | sc-32275 AF680 | 200 µg/ml | $364.00 | |||
p-Tau Antibody (PHF-13) Alexa Fluor® 790 | sc-32275 AF790 | 200 µg/ml | $364.00 |