p-Ribosomal Protein S6 Antibody (50.Ser 235/236): sc-293144

p-Ribosomal Protein S6 Antibody (50.Ser 235/236) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphorylated Ribosomal Protein S6 at Ser 235 and Ser 236 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Ribosomal Protein S6 antibody (50.Ser 235/236) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Ribosomal Protein S6, encoded by the RPS6 gene, plays a crucial role in regulating protein synthesis and cell growth, making RPS6 a key player in cellular responses to growth factors and mitogenic signals. Phosphorylation of RPS6 is particularly important as RPS6 serves as a major substrate for protein kinases in eukaryotic ribosomes, and RPS6 modification is associated with activation of signaling pathways that promote cell proliferation and survival. RPS6 has been shown to interact with several other proteins involved in ribosome biogenesis and translation, including mTOR (mechanistic target of rapamycin), which is a central regulator of cell growth and metabolism. Conservation of RPS6 structure and function across evolutionarily distant species, such as yeast and humans, underscores RPS6′s fundamental role in cellular physiology. Understanding RPS6 phosphorylation dynamics and interactions can provide valuable insights into mechanisms of cancer and other diseases characterized by dysregulated cell growth.

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p-Ribosomal Protein S6 Antibody (50.Ser 235/236) References:

1. Ribosomal protein S6 cDNA from two Aedes mosquitoes encodes a carboxyl-terminal extension that resembles histone H1 proteins.  |  Hernandez, VP. and Fallon, AM. 1999. Genetica. 106: 263-7. PMID: 10897799
2. Antitumor efficacy of intermittent treatment schedules with the rapamycin derivative RAD001 correlates with prolonged inactivation of ribosomal protein S6 kinase 1 in peripheral blood mononuclear cells.  |  Boulay, A., et al. 2004. Cancer Res. 64: 252-61. PMID: 14729632
3. The mapping of seven intron-containing ribosomal protein genes shows they are unlinked in the human genome.  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
4. A ribosomal protein S6 kinase copurifies with phosphatase-1 activating factor.  |  Khatra, BS., et al. 1991. Arch Biochem Biophys. 284: 186-92. PMID: 1989496
5. TPA-induced cell transformation provokes a complex formation between Pin1 and 90 kDa ribosomal protein S6 kinase 2.  |  Cho, YS., et al. 2012. Mol Cell Biochem. 367: 85-92. PMID: 22562304
6. Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe.  |  Gross, T., et al. 1988. Curr Genet. 13: 57-63. PMID: 2834104
7. Isolation and characterization of cloned cDNAs that code for human ribosomal protein S6.  |  Lott, JB. and Mackie, GA. 1988. Gene. 65: 31-9. PMID: 2840355
8. The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.  |  Heinze, H., et al. 1988. J Biol Chem. 263: 4139-44. PMID: 3279029
9. Comparative studies on phosphorylation of synthetic peptide analogue of ribosomal protein S6 and 40-S ribosomal subunits between Ca2+/phospholipid-dependent protein kinase and its protease-activated form.  |  Sakanoue, Y., et al. 1987. Eur J Biochem. 168: 669-77. PMID: 3311752
10. Identification of 40 S ribosomal protein S6 phosphorylation sites in Swiss mouse 3T3 fibroblasts stimulated with serum.  |  Bandi, HR., et al. 1993. J Biol Chem. 268: 4530-3. PMID: 8440735

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Ribosomal Protein S6 Antibody (50.Ser 235/236) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Ribosomal Protein S6 Antibody (50.Ser 235/236) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Ribosomal Protein S6 Antibody (50.Ser 235/236) Specifications

Alternate Product Name: Ribosomal Protein S6

Product Clone Name: 50.Ser 235/236

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 235/Ser 236

Epitope Target: Ribosomal Protein S6

Epitope Species: human

Product Reactivity/Detection: Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for p-Ribosomal Protein S6 Antibody (50.Ser 235/236): AC: Agarose, HRP: Horseradish Peroxidase

The preferred Ribosomal Protein S6 antibody is Ribosomal Protein S6 Antibody (C-8): sc-74459.