p-PP2A-Cα/β Antibody (F-8): sc-271903

p-PP2A-Cα/β Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-PP2A-Cα/β across multiple species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-PP2A-Cα/β (F-8) antibody comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. The protein phosphatase 2A (PP2A) catalytic subunit regulates protein phosphorylation states, maintaining cellular homeostasis and controlling cell division and apoptosis. Phosphorylation of Tyr 307 on PP2A-C isoforms by receptor and non-receptor protein tyrosine kinases inactivates phosphatase activity, influencing downstream signaling pathways. This temporary deactivation enhances signal transmission through kinase cascades, demonstrating PP2A′s significance in cellular functions. Several kinases, including p60v-src and epidermal growth factor receptors, catalyze PP2A phosphorylation, while phosphatase inhibitors like okadaic acid can increase phosphorylation levels. Understanding PP2A phosphorylation dynamics helps reveal roles in physiological and pathological processes, making anti-p-PP2A-Cα/β antibody (F-8) essential for cellular signaling research.

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p-PP2A-Cα/β Antibody (F-8) References:

1. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation.  |  Chen, J., et al. 1992. Science. 257: 1261-4. PMID: 1325671
2. Structure and expression of two isoforms of the murine calmodulin-dependent protein phosphatase regulatory subunit (calcineurin B).  |  Ueki, K., et al. 1992. Biochem Biophys Res Commun. 187: 537-43. PMID: 1325794
3. Phosphorylated PP2A (tyrosine 307) is associated with Alzheimer neurofibrillary pathology.  |  Liu, R., et al. 2008. J Cell Mol Med. 12: 241-57. PMID: 18208556
4. NXNL1 negatively regulates osteoblast differentiation via GDF15-induced PP2A Cα dependent manner in MC3T3-E1 cells.  |  Kim, KM. and Jang, WG. 2022. Biofactors. 48: 239-248. PMID: 34932831
5. Mitochondria-targeted catalase induced cell malignant transformation by the downregulation of p53 protein stability via USP28/miR-200b/PP2A-Cα axis.  |  Wen, C., et al. 2024. Arch Biochem Biophys. 758: 110047. PMID: 38844154
6. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293
7. Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts.  |  Chen, J., et al. 1994. J Biol Chem. 269: 7957-62. PMID: 7510677
8. Serine/threonine protein phosphatases.  |  Wera, S. and Hemmings, BA. 1995. Biochem J. 311 (Pt 1): 17-29. PMID: 7575450
9. Important roles for novel protein phosphatases dephosphorylating serine and threonine residues.  |  Cohen, PT. 1993. Biochem Soc Trans. 21: 884-8. PMID: 8132087
10. Protein serine/threonine phosphatases: structure, regulation, and functions in cell growth.  |  Mumby, MC. and Walter, G. 1993. Physiol Rev. 73: 673-99. PMID: 8415923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PP2A-Cα/β Antibody (F-8) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PP2A-Cα/β Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PP2A-Cα/β Antibody (F-8) Specifications

Alternate Product Name: p-PP2A phosphatase catalytic subunit C alpha/beta

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 307

Epitope Target: PP2A-Cα

Epitope Species: mouse

Product Reactivity/Detection: Tyr 307 phosphorylated PP2A-C α and β isoforms

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for p-PP2A-Cα/β Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin