p-PKA IIα reg Antibody (C-5): sc-377575

p-PKA IIα reg Antibody (C-5) is a mouse monoclonal IgM kappa antibody that detects Ser 96 phosphorylated PKAα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-PKA IIα reg (C-5) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. PKAα plays a crucial role in cellular signaling pathways, particularly in the context of the second messenger cyclic AMP (cAMP), which is essential for mediating a wide range of physiological responses, including cell proliferation, ion transport, metabolic regulation, and gene transcription. Upon binding of cAMP to the regulatory subunits of the tetrameric PKA holoenzyme, the active catalytic subunits are released, allowing PKA to phosphorylate target proteins, including the transcription factor cAMP response element binding protein (CREB). This phosphorylation event facilitates the recruitment of transcriptional machinery to initiate gene expression, thereby linking extracellular signals to cellular responses. Understanding PKAα function and regulatory mechanisms is important for elucidating pathways involved in various diseases, including cancer and metabolic disorders, making p-PKA IIα reg (C-5) antibody a valuable research tool.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-PKA IIα reg Antibody (C-5) References:

1. CDK1-mediated phosphorylation of the RIIalpha regulatory subunit of PKA works as a molecular switch that promotes dissociation of RIIalpha from centrosomes at mitosis.  |  Carlson, CR., et al. 2001. J Cell Sci. 114: 3243-54. PMID: 11591813
2. Regulation of anchoring of the RIIalpha regulatory subunit of PKA to AKAP95 by threonine phosphorylation of RIIalpha: implications for chromosome dynamics at mitosis.  |  Landsverk, HB., et al. 2001. J Cell Sci. 114: 3255-64. PMID: 11591814
3. A novel CK2-mediated activation of type II cAMP-dependent protein kinase through specific phosphorylation of its regulatory subunit (RIIalpha) in vitro.  |  Kosuge, S., et al. 2003. Biochem Biophys Res Commun. 310: 163-8. PMID: 14511665
4. Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase.  |  Beebe, SJ., et al. 1990. Mol Endocrinol. 4: 465-75. PMID: 2342480
5. Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133.  |  Gonzalez, GA. and Montminy, MR. 1989. Cell. 59: 675-80. PMID: 2573431
6. Anchoring of both PKA-RIIα and 14-3-3θ regulates retinoic acid induced 16 mediated phosphorylation of heat shock protein 70.  |  Ding, CL., et al. 2015. Oncotarget. 6: 15540-50. PMID: 25900241
7. A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha.  |  Maldonado, F. and Hanks, SK. 1988. Nucleic Acids Res. 16: 8189-90. PMID: 2843813
8. Phosphorylation-dephosphorylation of enzymes.  |  Krebs, EG. and Beavo, JA. 1979. Annu Rev Biochem. 48: 923-59. PMID: 38740
9. Activation of protein kinase by physiological concentrations of cyclic AMP.  |  Beavo, JA., et al. 1974. Proc Natl Acad Sci U S A. 71: 3580-3. PMID: 4372627
10. Signal transduction through the cAMP-dependent protein kinase.  |  Meinkoth, JL., et al. 1993. Mol Cell Biochem. 127-128: 179-86. PMID: 7935349
11. Transcription factors. CREB takes CBP to tango.  |  Nordheim, A. 1994. Nature. 370: 177-8. PMID: 8028657
12. Mitosis-specific phosphorylation and subcellular redistribution of the RIIalpha regulatory subunit of cAMP-dependent protein kinase.  |  Keryer, G., et al. 1998. J Biol Chem. 273: 34594-602. PMID: 9852131

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKA IIα reg Antibody (C-5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKA IIα reg Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKA IIα reg Antibody (C-5) Specifications

Alternate Product Name: PKA IIα reg

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 96

Epitope Target: PKAα

Epitope Species: mouse

Product Reactivity/Detection: Ser 96 phosphorylated PKAα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for p-PKA IIα reg Antibody (C-5): P: Blocking Peptide

The preferred PKA IIα reg antibody is PKA IIα reg Antibody (H-12): sc-137220.