p-PDGF Receptor beta/PDGFRB Antibody (F-10): sc-365464

p-PDGFR-β Antibody (F-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects phosphorylated PDGF receptor beta (p-PDGFR-β) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-PDGFR-β (F-10) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. The platelet-derived growth factor (PDGF) receptor beta plays a crucial role in cellular processes such as proliferation, survival, and migration, particularly in mesenchymal and glial cells. PDGFR-β is primarily located on the cell surface, where PDGFR-β interacts with PDGF-AA, PDGF-AB, and PDGF-BB ligands, leading to receptor dimerization and activation of intrinsic tyrosine kinase activity. This activation results in autophosphorylation at key tyrosine residues, including Tyr 857 and Tyr 751, which are essential for downstream signaling pathways. Notably, phosphorylation of Tyr 751 is critical for recruiting phosphatidylinositol-3 kinase, a key player in the signaling cascade that promotes cell survival and growth. Understanding p-PDGFR-β signaling dynamics is vital for elucidating p-PDGFR-β′s role in various physiological and pathological processes, including tissue repair and tumorigenesis.

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p-PDGF Receptor beta/PDGFRB Antibody (F-10) References:

1. Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1.  |  Kovalenko, M., et al. 2000. J Biol Chem. 275: 16219-26. PMID: 10821867
2. Treatment with Imatinib in NSCLC is associated with decrease of phosphorylated PDGFR-beta and VEGF expression, decrease in interstitial fluid pressure and improvement of oxygenation.  |  Vlahovic, G., et al. 2006. Br J Cancer. 95: 1013-9. PMID: 17003785
3. Platelet-derived growth factor (PDGF) stimulates PDGF receptor subunit dimerization and intersubunit trans-phosphorylation.  |  Kelly, JD., et al. 1991. J Biol Chem. 266: 8987-92. PMID: 1709159
4. Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins.  |  Kazlauskas, A. and Cooper, JA. 1989. Cell. 58: 1121-33. PMID: 2550144
5. Two classes of PDGF receptor recognize different isoforms of PDGF.  |  Hart, CE., et al. 1988. Science. 240: 1529-31. PMID: 2836952
6. Binding of different dimeric forms of PDGF to human fibroblasts: evidence for two separate receptor types.  |  Heldin, CH., et al. 1988. EMBO J. 7: 1387-93. PMID: 2842148
7. The biology of platelet-derived growth factor.  |  Ross, R., et al. 1986. Cell. 46: 155-69. PMID: 3013421
8. Stromal integrin α11 regulates PDGFR-β signaling and promotes breast cancer progression.  |  Primac, I., et al. 2019. J Clin Invest. 129: 4609-4628. PMID: 31287804
9. A unique autophosphorylation site in the platelet-derived growth factor alpha receptor from a heterodimeric receptor complex.  |  Rupp, E., et al. 1994. Eur J Biochem. 225: 29-41. PMID: 7523122
10. Two signaling molecules share a phosphotyrosine-containing binding site in the platelet-derived growth factor receptor.  |  Nishimura, R., et al. 1993. Mol Cell Biol. 13: 6889-96. PMID: 7692233

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PDGF Receptor beta/PDGFRB Antibody (F-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PDGF Receptor beta/PDGFRB Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PDGF Receptor beta/PDGFRB Antibody (F-10) Specifications

Alternate Product Name: PDGFRB

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Tyr 716

Epitope Target: PDGFR-β

Epitope Species: human

Product Reactivity/Detection: Tyr 716 phosphorylated PDGFR-β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for p-PDGF Receptor beta/PDGFRB Antibody (F-10): P: Blocking Peptide

The preferred PDGF Receptor beta/PDGFRB antibody is PDGF Receptor beta/PDGFRB Antibody (D-6): sc-374573.